Record Details

Capsicum annuum proteinase inhibitor ingestion negatively impacts the growth of sorghum pest Chilo partellus and promotes differential protease expression

MELSpace

View Archive Info
 
 
Field Value
 
Title Capsicum annuum proteinase inhibitor ingestion negatively impacts the growth of sorghum pest Chilo partellus and promotes differential protease expression
 
Creator Jadhav, Abhilash R.
 
Contributor War, Abdul Rashid
Nikam, Ashwini
Adhav, Anmol
Gupta, Vidya S.
Sharma, Hari C.
Giri, Ashok P.
Tamhane, Vaijayanti A.
 
Subject plant-insect interaction
sorghum pests
protease up-regulation
Sorghum
 
Description Background: Chilo partellus is an important insect pest infesting sorghum and maize. The larvae internalize in
the stem, rendering difficulties in pest management. We investigated the effects of Capsicum annuum
proteinase inhibitors (CanPIs) on C. partellus larvae by in-vitro and in-vivo experiments.
Methods: Recombinant CanPI-7 (with four-Inhibitory Repeat Domains, IRDs), -22 (two-IRDs) and insect
proteinase activities were estimated by proteinase assays, dot blot assays and in gel activity assays. Feeding
bioassays of lab reared C. partellus with CanPI-7 and -22 were performed. C. partellus proteinase gene
expression was done by RT-PCR. In-silico structure prediction of proteinases and CanPI IRDs was carried out,
their validation and molecular docking was done for estimating the interaction strength.
Results: Larval proteinases of C. partellus showed higher activity at alkaline pH and expressed few proteinase
isoforms. Both CanPIs showed strong inhibition of C. partellus larval proteinases. Feeding bioassays of C.
partellus with CanPIs revealed a dose dependent retardation of larval growth, reduction of pupal mass and
fecundity, while larval and pupal periods increased significantly. Ingestion of CanPIs resulted in differential upregulation
of C. partellus proteinase isoforms, which were sensitive to CanPI-7 but were insensitive to CanPI-
22. In-silico interaction studies indicated the strong interaction of IRD-9 (of CanPI-22) with Chilo proteinases
tested.
Conclusions: Of the two PIs tested, CanPI-7 prevents induction of inhibitor insensitive proteinases in C.
partellus so it can be explored for developing C. partellus tolerance in sorghum.
General significance: Ingestion of CanPIs, effectively retards C. partellus growth; while differentially regulating
the proteinases.
 
Date 2016-10-06
2017-03-22T02:14:11Z
2017-03-22T02:14:11Z
 
Type Journal Article
 
Identifier http://oar.icrisat.org/id/eprint/9752
https://mel.cgiar.org/reporting/download/hash/GPZ6SW3X
Abhilash R. Jadhav, Abdul Rashid War, Ashwini Nikam, Anmol Adhav, Vidya S. Gupta, Hari C. Sharma, Ashok P. Giri, Vaijayanti A. Tamhane. (6/10/2016). Capsicum annuum proteinase inhibitor ingestion negatively impacts the growth of sorghum pest Chilo partellus and promotes differential protease expression. Biochemistry and Biophysics Reports, 8, pp. 302-309.
https://hdl.handle.net/20.500.11766/6522
Open access
 
Language en
 
Rights CC-BY-NC-4.0
 
Format PDF
 
Publisher Elsevier
 
Source Biochemistry and Biophysics Reports;8,(2016) Pagination 302,309