| Abstract |
The course will introduce participants to the basics of plant genome analysis and its application to plant breeding problems in both wheat and maize through lectures and hands-on exercises. Participants will learn the isolation, digestion, electrophoresis and transfer of DNA to blots, the non-radioactive RFLP technology now in use at CIMMYT, the generation of PCR-based markers and AFLPs, bacterial transformation and plasmid preparation. Participants will also enter and verify RFLP data into the computer, analyse data to produce genetic maps, and perform QTL analyses with different st~tistical tools. In addition, there will be an introduction to the public databases available for maize and the Triticeae and their uses. Opportunities will be provided to discuss the application of molecular markers to plant breeding, the interpretation of data from molecular analyses and the research that each participant is involved in as it relates to the course. |
ICAR Research Data Repository for Knowledge Management