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Title Optimization of an in vitro bioassay for wheat diseases
 
Names Salgado, M.M.
Pellegrineschi, A.
Mezzalama, M.
McLean, S.
Hoisington, D.A.
Date Issued 2000 (iso8601)
Abstract Alternaria triticina, Fusarium graminearum, Pyrenophora tritici-repentis, Blpolaris sorokinisna (Helminthosporium sativum); Pythium sp., and Rhizoctonia sp. were tested on the basis of being a representative group of important fungal pathogens in order to establish a reliable bloassay system to test eventual fungal resistance in transgenic plants. The inoculum was prepared on V-8 agar medium (in petri dishes) for P. tritici-repents and Pythlum sp., and on PDA (potato dextrose agar) medium for the other fungi. The cultures were incubated at room temperature for 7- 10 days in a culture chamber with a constant standard illumination. The suspensions of conidia or micella were prepared on sterile distilled water with 1 few drops of tween 20 and scrapings of the fungi culture. The inoculum was homogenized by vorlexing the suspension for a few seconds. The concentration used for the conidia solution was adjusted to 106 conidia/ml. Fresh leaf samples from adult plants (heading stage) were sterilized and then dipped In the inoculum suspension. The inoculated leaves were then transferred to water agar medium (1% agar), in 8-well rectangular multi-dishes, at room temperature. The level of resistance of the material to these pathogens was evaluated 4-7 days after inoculation. We believe that the routine use of this protocol (fast, reliable, and inexpensive) could allow the easy identification of resistant/tolerant plants to these diseases. In addition, because of the simplicity of this test, it can be useful for early screening of a large number of individuals, as required for transgenic plant screenings.
Genre Newsletter / Bulletin
Access Condition Open Access
Identifier http://hdl.handle.net/10883/3940