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Genetic characterization and protein profiling of green mussel (Perna viridis (Linnaeus, 1758)) and brown mussel (Perna indica Kuriakose and Nair, 1976)

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Relation http://eprints.cmfri.org.in/8912/
 
Title Genetic characterization and protein profiling of green mussel (Perna viridis (Linnaeus, 1758)) and brown mussel (Perna indica Kuriakose and Nair, 1976)
 
Creator Divya, P R
 
Subject Fish Genetics
Microbiology
Theses
Bioactive compound
 
Description The marine mussels of India are mainly of two species: Perna viridis
[Linnaeus, 1758) (green mussel) and Perna indica Kuriakose and Nair (1976)
[brown mussel). In addition to this, along the south west coast (Kollam coast
f Kerala), a third type of mussel called parrot mussel has also been reported.
The present study was undertaken for genetic characterisation and
dentification of distinct stocks if any, within green as well as brown mussels,
From both east and west coast, using shell morphometrics and molecular
markers. Stock identification using shell morphometrics were attempted with
Principal Component Analysis and Canonical Discriminant function Analysis,
whereas, molecular methods used were protein electrophoresis (Native - Poly
Acrylamide Gel Electrophoresis) and Randomly Amplified Polymorphic DNAPolymerase
Chain Reaction (RAPD-PCR). The shell morphometrics as well
as Native PAGE and RAPD-PCR profiles gave concordant results indicating
high intraa species homogeneity and low level of genetic differentiation among
populations of both green and brown mussels. Genetic characterisation using
RAPD markers could bring out the genetic variability within both the
populations. Elucidation of the genetic identity of parrot mussel as to whether
it is a true hybrid of green and brown mussel, or only a colour variant of any of
them was also done using RAPD, shell morphometrics, protein
electrophoresis (native PAGE, SDS (Sodium Dodecyl Sulphate) PAGE and
allozymes) and mitochondria1 (mt) DNA gene (Cytochrome oxidase 1)
analysis. The study concluded that the parrot mussel is not a hybrid of the
two, but only a colour variant of the brown mussel. An initial attempt to resolve
the taxonomic ambiguity of P.indica that it is only a synonym of globally
distributed Perna perna, as suggested by some of the previous workers has
also been made in this study using COI regions of mt DNA. As P. indica
revealed only 5% divergence from P, perna, it was concluded that P. indica
need not be relegated as a synonym of P.perna
 
Date 2008
 
Type Thesis
NonPeerReviewed
 
Format application/pdf
 
Language en
 
Identifier http://eprints.cmfri.org.in/8912/1/THESIS_161.pdf
Divya, P R (2008) Genetic characterization and protein profiling of green mussel (Perna viridis (Linnaeus, 1758)) and brown mussel (Perna indica Kuriakose and Nair, 1976). ["eprint_fieldopt_thesis_type_phd" not defined] thesis, Central Marine Fisheries Research Institute. Submitted to Central Institute of Fisheries Education.