Replication data for: Multilevel Agrobiodiversity and Conservation of Andean Potatoes in Central Peru
International Potato Center Dataverse OAI Archive
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Title |
Replication data for: Multilevel Agrobiodiversity and Conservation of Andean Potatoes in Central Peru
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Identifier |
https://doi.org/10.21223/P3/681TF7
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Creator |
De Haan, Stef
Nuñez, Jorge Bonierbale, Merideth Ghislain, Marc |
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Publisher |
International Potato Center
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Description |
A population consisting of 989 samples belonging to 8 farmer households (ISC-01 to ISC-08) was characterized using 18 polymorphic single sequence repeat (SSR; microsatellite) markers covering the whole genome (Ghislain et al 2004). Standard procedures, including DNA extraction with DNeasy 96 plant kits, high-throughput genotyping with a LI-COR 4300 DNA Analysis System,and SSR allele scoring with SAGA Generation 2 software (LI-COR), were applied. Standardized dissimilarity analysis for ISC-01 to ISC-08 and the regional population were conducted using the Jaccard coefficient and UPGMA clustering method with NTSYS-pc 2.1 software. Dissimilarity trees (dendrograms) were built with the same data using an unweighted neighbor joining clustering method for a dissimilarity matrix calculated with the Jaccard coefficient using DARwin 4.0 and NTSYS-pc 2.1 software.
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Subject |
Agricultural Sciences
Potato In situ conservation Ploidy Native cultivars Molecular markers Single sequence repeat (SSR) markers Huancavelica Peru |
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Language |
English
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Contributor |
Administrator, CIP
CGIAR Research Program on Roots, Tubers and Bananas (RTB) National Institute for Agriculture and Food Research and Technology (INIA), Spain Alberto Salas Rene Gomez International Potato Center |
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Type |
xls
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