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Replication data for: Multilevel Agrobiodiversity and Conservation of Andean Potatoes in Central Peru

International Potato Center Dataverse OAI Archive

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Title Replication data for: Multilevel Agrobiodiversity and Conservation of Andean Potatoes in Central Peru
 
Identifier https://doi.org/10.21223/P3/681TF7
 
Creator De Haan, Stef
Nuñez, Jorge
Bonierbale, Merideth
Ghislain, Marc
 
Publisher International Potato Center
 
Description A population consisting of 989 samples belonging to 8 farmer households (ISC-01 to ISC-08) was characterized using 18 polymorphic single sequence repeat (SSR; microsatellite) markers covering the whole genome (Ghislain et al 2004). Standard procedures, including DNA extraction with DNeasy 96 plant kits, high-throughput genotyping with a LI-COR 4300 DNA Analysis System,and SSR allele scoring with SAGA Generation 2 software (LI-COR), were applied. Standardized dissimilarity analysis for ISC-01 to ISC-08 and the regional population were conducted using the Jaccard coefficient and UPGMA clustering method with NTSYS-pc 2.1 software. Dissimilarity trees (dendrograms) were built with the same data using an unweighted neighbor joining clustering method for a dissimilarity matrix calculated with the Jaccard coefficient using DARwin 4.0 and NTSYS-pc 2.1 software.
 
Subject Agricultural Sciences
Potato
In situ conservation
Ploidy
Native cultivars
Molecular markers
Single sequence repeat (SSR) markers
Huancavelica
Peru
 
Language English
 
Contributor Administrator, CIP
CGIAR Research Program on Roots, Tubers and Bananas (RTB)
National Institute for Agriculture and Food Research and Technology (INIA), Spain
Alberto Salas
Rene Gomez
International Potato Center
 
Type xls