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Dataset for: Potato seed degeneration and soil colonization driven by inoculum sources of Rhizoctonia solani

International Potato Center Dataverse OAI Archive

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Title Dataset for: Potato seed degeneration and soil colonization driven by inoculum sources of Rhizoctonia solani
 
Identifier https://doi.org/10.21223/P3/DRJSRL
 
Creator Navarrete, Israel
Kromann, Peter
Andrade-Piedra, Jorge
Thomma, Bart
Struik, Paul
 
Publisher International Potato Center
 
Description Rhizoctonia solani is a saprophytic fungal pathogen causing potato seed degeneration. Unfortunately, the effects on seed degeneration and soil colonization caused by R. solani inoculum sources (black scurf on seed tubers and propagules in the soil), and inoculum densities have been poorly quantified. For this reason, we carried out a research aiming to: (1) understand the role of inoculum sources and densities in potato seed degeneration, and (2) understand the role of the inoculum sources and densities in soil colonization by the fungus. For objective 1, a glasshouse experiment was performed in Wageningen, from March to July 2015 (glasshouse temperature and relative humidity are described in the "Glasshouse_conditions" sheet, and soil temperature and moisture content are described in the "Soil_conditions" sheet). Two factors were studied: inoculum sources and densities (for seed tubers: 0%, 0-5%, 5-10%, 10-15%, and more than 15% of black scurf; and for soil: 0, 8, and 16 propagules / 100 g of dry weight of soil), leading to the establishment of 15 treatments in this experiment. This experiment was replicated three times and assessed between 3 to 6 plants planted in 5L pots per treatment and replication. Seed tubers (var. Carolus) with black scurf were selected using keys, and soil was inoculated using a Dutch isolate of R. solani AG-3. Variables of plant development, productivity and infection were recorded and detailed in the “Glasshouse_experimental_data” sheet. For objective 2, R. solani DNA was extracted from 5g soil samples taken from the pots at 30, 60, and approximately 100 days after planting (~100). Soil samples of six treatments (a composite sample per replicate) were selected, in which, seed tubers with 0%, 0-5%, and >15% of black scurf were planted; and 0 and 16 propagules / 100 g of dry weight of soil were inoculated. The R. solani DNA amount in the soil samples was quantified using a real-time PCR (“Laboratory_experimental_data” sheet) and calibration curves based on serial dilutions of known R. solani DNA concentrations (details in the “Calibration_data” sheet).
 
Subject Agricultural Sciences
Black scurf
Inoculum sources
Inoculum density
DNA quantification
 
Language English
 
Contributor Administrator, CIP
International Potato Center Quito
CGIAR Research Program on Roots, Tubers and Bananas (RTB)
The Mcknight foundation
Understanding Potato Seed Degeneration in Ecuador: A M.Sc. project
 
Type xls