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Replication data for: Complete genome sequence of a potyvirus infecting yam beans (Pachyrhizus spp.) in Peru

International Potato Center Dataverse OAI Archive

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Title Replication data for: Complete genome sequence of a potyvirus infecting yam beans (Pachyrhizus spp.) in Peru
 
Identifier https://doi.org/10.21223/P3/H05A0F
 
Creator Fuentes, Segundo
Heider, Bettina
Tasso, Ruby
Romero, Elisa
Zum Felde, Thomas
Kreuze, Jan
 
Publisher International Potato Center
 
Description Towards the end of 2010, viral disease symptoms (mosaic with or without leaf deformation) were observed in a yam bean field trial at the CIP research station in San Ramon, Junin, Peru (wet tropics, 800 masl). The disease could be graft transmitted, and based on symptoms, an infection by the potyvirus bean common mosaic virus (BCMV) was suspected. Plants were tested by NCM-ELISA using potyvirus-specific antibodies (Agdia CAB 27200), to which all symptomatic plants reacted positively, whereas symptomless greenhouse-grown plants did not react. Small interfering RNA (siRNA) sequencing (Provider: Fasteris Life Sciences SA, Plan-les-Ouates, Switzerland) and assembly were performed on a sample (P. tuberosus accession CIP209014) showing severe mosaic and leaf deformation as described by Kreuze et al., except that the reads were assembled using Velvet’s Assembly Assembler (v1.1)
 
Subject Agricultural Sciences
Yam beans
Genome sequence
Potyvirus
 
Language English
 
Contributor Administrator, CIP
CGIAR Research Program on Roots, Tubers and Bananas (RTB)
International Potato Center
Flores, Mirella
 
Type fastq