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Replication data for: Cryopreserved potato shoot tips showed genotype-specific response to sucrose concentration in rewarming solution (RS)

International Potato Center Dataverse OAI Archive

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Title Replication data for: Cryopreserved potato shoot tips showed genotype-specific response to sucrose concentration in rewarming solution (RS)
 
Identifier https://doi.org/10.21223/P3/JGBGZ0
 
Creator Vollmer, Rainer
Villagaray, Rosalva
Castro, Mario
Anglin, Noelle
Ellis, David
 
Publisher International Potato Center
 
Description In an experiment with 16 clonal potato accessions, the use of RS sucrose concentrations of 0.6 M and 0.9 M showed on average ~ 5% higher recovery rate, compared to the routinely used 1.2 M sucrose concentration, suggesting that sucrose can be decreased in RS. A screening experiment with a set of 85 genetically diverse potato accessions confirmed this suggestion, and sucrose concentrations of 0.3–0.9 M resulted in significantly higher recovery rates compared to the routinely used 1.2 M sucrose concentration. The genotypic specific response to low (0.0 M) and high RS sucrose concentrations (1.2 M) was variable within species/subspecies and ploidy levels. Interestingly, some genotypes showed high recovery rates of ≥80% even when sucrose was completely removed from the RS. In general, the highest recovery was observed with a RS sucrose concentration of 0.6 M (“best bet”), and therefore CIP’s routine cryopreservation protocol for potato has been changed accordingly (from 1.2 M to 0.6 M RS sucrose concentration). We recommend being cautious in generalizing experimental cryopreservation results obtained with a limited number of accessions to larger collections.
 
Subject Agricultural Sciences
Cryopreservation
Cryoprotectants
Potatoes
Survival
Regrowth/Recovery
Viability
Species
Sucrose
Thawing
 
Language English
 
Contributor Administrator, CIP
Global Crop Diversity Trust
Cryobanking at the International Potato Center
Genebank CGIAR Research Program
 
Type XLS