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Some immunobiological aspects of the spiny lobster Panulirus homarus (Linnaeus, 1758)(TH 114)

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Relation http://eprints.cmfri.org.in/7165/
 
Title Some immunobiological aspects of the spiny lobster Panulirus
homarus (Linnaeus, 1758)(TH 114)
 
Creator Verghese, Bindhu
 
Subject Theses
Lobsters
 
Description Panulirus homarus (Linnaeus, 1758) being a species of great commercial
importance fetches high demand in the international market. The demand of live
lobsters depends on the health status of the animals. Study on the defence
mechanisms in P. homarus showed three cell types in the haemolymph: hyaline
cells, semi granular cells and large granular cells. The haemocy1es are produced
from the haematopoetic tissue located in the epigastric region, they are lobular
with a size range of 30-875 jJm. The immune status of healthy lobsters showed
that the total haemocy1e count was 8.7 ± 3.8 x 106 cell mr l and the phenoloxidase
activity 30.61 ± 5.6 IU mg protein- 1
• Studies on physiological stress like moulting,
eyestalk ablation showed variation in the immune parameters (P < 0.05) . Upon
starvation the THe and PO activity was found to decrease drastically. Feed trials
showed that mussels, the preferred diet of lobsters was found to show better
immune response over those fed with fish diet. Environmental stress factors like
salinity, pH, dissolved oxygen and ammonia showed significant effect on the
immune system of P. homarus. Live transportation of lobsters by dry method was
found to suppress the PO activity but no change was seen in THe. Injection
studies with bacteria showed the lowest peak during 30 minutes incubation for
THe whereas it showed highest peak for PO activity for the same incubation time.
Both THe and PO levels came back to normal after 24 hours. On injection with
laminarin an immunostimulant similar result was obtained. Farm made artificial
feed supplemented with chitosan was found to enhance the activity of the immune
system . Satisfactory result for immune enhancement was not observed with
commercial immunostimulant supplement. Analysis of serum PO activity and total
haemocy1e count can be used as indicators of the health in crustaceans, which
can be monitored, even in farm conditions. Routine monitoring of health status is
of great importance with regard to successful aquaculture practices.
 
Date 2003
 
Type Thesis
NonPeerReviewed
 
Format application/pdf
 
Language en
 
Identifier http://eprints.cmfri.org.in/7165/1/TH-114.pdf
Verghese, Bindhu (2003) Some immunobiological aspects of the spiny lobster Panulirus homarus (Linnaeus, 1758)(TH 114). ["eprint_fieldopt_thesis_type_phd" not defined] thesis, PGPM, Central Marine Fisheries Research Institute.