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Replication data for: Complete viral genome sequence and discovery of novel viruses by deep sequencing of small RNAs: A generic method for diagnosis, discovery and sequencing of viruses

International Potato Center Dataverse OAI Archive

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Title Replication data for: Complete viral genome sequence and discovery of novel viruses by deep sequencing of small RNAs: A generic method for diagnosis, discovery and sequencing of viruses
 
Identifier https://doi.org/10.21223/P3/NKWPGE
 
Creator Kreuze, Jan
Perez, Ana
Untiveros, Milton
Quispe, Dora
Fuentes, Segundo
Barker, Ian
Simon, Reinhard
 
Publisher International Potato Center
 
Description Pathogen-tested in vitro plants of the Peruvian sweetpotato landrace ‘Huachano’ (accession no. CIP420065) were obtained from the germplasm collection of the International Potato Center (CIP, Lima, Peru) and transferred to an insect-proof greenhouse at CIP. The isolate of Sweetpotato feathery mottle virus SPFMV (SPFMV-Piu) and Sweetpotato chlorotic stunt virus SPCSV (SPCSV m2–47) used for plant inoculation in this study were originally obtained from sweetpotato plants infected in the field in Peru (Cuellar et al., 2008). They were maintained in I. setosa plants in an insect-proof greenhouse at CIP, Lima, Peru. The viruses were inoculated to sweetpotato plants via grafting. Infection was confirmed by index grafting onto the susceptible indicator plants of I. setosa and double or triple antibody sandwich enzyme linked immunosorbent assay for SPFMV and SPCSV respectively as described in Kreuze et al. (2008).
 
Subject Agricultural Sciences
siRNA
Deep sequencing
Viral diagnostics
Plant viruses
Virus discovery
Sweetpotato
Badnavirus
Mastrevirus
Potyvirus
Crinivirus
Short sequence assembly
 
Language English
 
Contributor Administrator, CIP
CGIAR Research Program on Roots, Tubers and Bananas (RTB)
Howard Buffet Foundation
Marc Ghislain
Roger Beachy
International Potato Center
 
Type fastq