Replication data for: Complete viral genome sequence and discovery of novel viruses by deep sequencing of small RNAs: A generic method for diagnosis, discovery and sequencing of viruses
International Potato Center Dataverse OAI Archive
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Title |
Replication data for: Complete viral genome sequence and discovery of novel viruses by deep sequencing of small RNAs: A generic method for diagnosis, discovery and sequencing of viruses
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Identifier |
https://doi.org/10.21223/P3/NKWPGE
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Creator |
Kreuze, Jan
Perez, Ana Untiveros, Milton Quispe, Dora Fuentes, Segundo Barker, Ian Simon, Reinhard |
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Publisher |
International Potato Center
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Description |
Pathogen-tested in vitro plants of the Peruvian sweetpotato landrace ‘Huachano’ (accession no. CIP420065) were obtained from the germplasm collection of the International Potato Center (CIP, Lima, Peru) and transferred to an insect-proof greenhouse at CIP. The isolate of Sweetpotato feathery mottle virus SPFMV (SPFMV-Piu) and Sweetpotato chlorotic stunt virus SPCSV (SPCSV m2–47) used for plant inoculation in this study were originally obtained from sweetpotato plants infected in the field in Peru (Cuellar et al., 2008). They were maintained in I. setosa plants in an insect-proof greenhouse at CIP, Lima, Peru. The viruses were inoculated to sweetpotato plants via grafting. Infection was confirmed by index grafting onto the susceptible indicator plants of I. setosa and double or triple antibody sandwich enzyme linked immunosorbent assay for SPFMV and SPCSV respectively as described in Kreuze et al. (2008).
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Subject |
Agricultural Sciences
siRNA Deep sequencing Viral diagnostics Plant viruses Virus discovery Sweetpotato Badnavirus Mastrevirus Potyvirus Crinivirus Short sequence assembly |
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Language |
English
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Contributor |
Administrator, CIP
CGIAR Research Program on Roots, Tubers and Bananas (RTB) Howard Buffet Foundation Marc Ghislain Roger Beachy International Potato Center |
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Type |
fastq
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