Record Details

Dataset for: Viral kinetics of 16 PoGV isolates in Phthorimaea operculella

International Potato Center Dataverse OAI Archive

View Archive Info
 
 
Field Value
 
Title Dataset for: Viral kinetics of 16 PoGV isolates in Phthorimaea operculella
 
Identifier https://doi.org/10.21223/P3/RQBOQT
 
Creator Hualla, Vilma
Sporleder, Marc
Zegarra, Octavio
Kroschel, Jurgen
 
Publisher International Potato Center
 
Description Viral kinetics is the study of viral multiplication in a host (P. operculella larvae) and viral production (amount of granules per harvested larva).
A stock solution of PoGV was prepared for each isolate, with a concentration of 109 granules/ml, from which a dilution was made with the factor 4:1 (3 of distilled water: 1 virus) and proceeded to inoculate discs that contained between 800 to 1000 eggs of P. operculella. These filter paper discs were placed in petri dishes, sealing the plates until the larvae hatched.
Then, 50 larvae were transferred with the help of a brush to 15 plastic containers of ½ of a liter for each isolation, these containers contained approximately 100g of potato variety peruanita, next they were taken to a growth chamber at 24°C.
The evaluations were carried out daily from the sixth day until the 21st day. For this, five larvae of potato tubers were recovered, each larva was placed in a 1.5 ml microtube (eppendorf) and weighed immediately, to avoid weight loss.
After, the larvae were crushed in 1 ml of distilled water to count the occlusion bodies. The necessary dilutions were made to perform an optimal count, then 10 μl of the dilution was added to the Neubauer chamber and observed in a phase contrast microscope (Olympus).
 
Subject Agricultural Sciences
Granulovirus PoGV
Viral multiplication
Phthorimaea operculella
Potatoes
 
Language English
 
Contributor Administrator, CIP
CGIAR Research Program on Roots, Tubers and Bananas (RTB)
International Potato Center