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Nutritional studies on sebae Anemonefish, Amphiprion sebae Bleeker 1853, with special reference To protein and lipid requirements

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Relation http://eprints.cmfri.org.in/7221/
 
Title Nutritional studies on sebae
Anemonefish, Amphiprion sebae
Bleeker 1853, with special reference
To protein and lipid requirements
 
Creator Binu, Varghese
 
Subject Fish Biotechnology
Theses
Ornamental Fishes
 
Description Anemonefishes or clownfishes command higher price than most other pomacentrids
in the marine aquarium fish trade. Commercial production of these reef fishes can
generate valuable foreign exchange for the country. The sebae anemonefisi1,
Amphiprion sebae Bleeker 1853, is one of the popular marine aquarium fish . At
present rearing of the fish relies heavily on live and fresh diets, and formulated diets
are seldom used due to inadequate knowledge of the nutritional needs of the different
life-history stages. The present study focuses on the nutrition of broodstock, larvae
and juvenile stages of the fish in captivity. In order to identify suitable diets for brood
fishes the efficacy of six natural and five formulated diets was determined. The
natural diets tested were deep sea shrimp, cuttlefish, squid, mature mussel, mussel
meat, and a mixed diet of polychaete worm and mussel meat (1 :1). Among the
natural diets cuttlefish meat gave significantly higher number of eggs per spawning
(1521±264 eggs; mean ± SO, n=9) followed by the deep sea shrimp (1300±445
eggs). Among Ihe five formulated semi-moist diets used, diet CBOs with about 50%
protein and 10% lipid, gave significantly higher number (2137±110) of eggs pHr
spawning followed by diet CB02 (1683±436 eggs) which had 40% protein and 20%
lipid. The number of eggs per spawning was found to decrease significantly when
broodfish were fed ad libitum once a day (416128 eggs) compared to those fed ad
libitum twice daily (885±55 eggs). Hatchery reared larvae weaned to a dry diet from
3rd week onwards matured and the broodfish spawned successfully in aquaria from
15th month (age of fish) onwards. Feeding experiments using exclusive or
combinations of rotifers, Artemia, and Moina showed better survival when the larvae
were reared with micro algae and fed rotifers and Artemia. Weaning studies revealed
that weaning from livefeed to a dry microbound particulate diet after 3 weeks (21dph)
did not Significantly affect survival, though the best age of weaning was found to be
30 days post hatching. Experiments with a purified and semi-purified diet having 50%
protein and 6% lipid showed semi-purified diets to be better than purified diets.
Analysis of data from protein requirement experiments using semi-purified isocaloric
diets showed 46.2% as protein requirement for maximum weight gain in juveniles and
44.9% in terms of SGR. Lipid requirement experiment on juveniles using five
isoproteic semi-purified diets showed 10.96% as lipid requirement for maximum
weight gain and 11 .05% in terms of SGR. The present study proved the effectiveness
of formulated diets in rearing larvae, juveniles and brood stock of the clownfish. The
results are significant with enormous application in commercial production of these
high value fishes and in promoting indigenous aquarium feed manufacture.
 
Date 2004
 
Type Thesis
NonPeerReviewed
 
Format application/pdf
 
Language en
 
Identifier http://eprints.cmfri.org.in/7221/1/TH-126.pdf
Binu, Varghese (2004) Nutritional studies on sebae Anemonefish, Amphiprion sebae Bleeker 1853, with special reference To protein and lipid requirements. ["eprint_fieldopt_thesis_type_phd" not defined] thesis, Central Institute of Fisheries Education, Mumbai.