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Induction and evaluation of triploidy in Crassostrea madrasensis (Preston)(TH 135)

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Relation http://eprints.cmfri.org.in/7251/
 
Title Induction and evaluation of triploidy
in Crassostrea madrasensis (Preston)(TH 135)
 
Creator Mallia, Jyothi V
 
Subject Fish Biotechnology
Theses
 
Description The kinetics of extrusion of polarbody is essential
to produce triploids. Hence, studies were conducted on polarbody
extrusion at 22, 28 and 31(±I)OC. At 31,28 and 23°C, 50% of I
polar body was fonned 15, 16 and 18 minutes respectively after
fertilization. At 31,28 and 23oC, 50% of II polar body was fonned
at 28, 32 and 36 minutes respectively after fertilization. Hence
triploid induction treatments were administered 8 minutes after
fertilization for producing I meiotic triploids and 17 minutes after
fertilization for II meiotic triploids.
Among physical methods of induction for triploidy,
cold and heat shock treatments were applied. Cold shock at 5°C
for 10 minutes duration yielded 42.25% of triploidy.
High percentages 42.0 and 41.85 of triploids
occurred at 37°C for heat treatment, for 5 and 10 minutes,
respectively.
In the chemical treatment, among three
concentrations of cytochalasin B (0 .05, 0.10 and 0.15 mg/I).
40.0% triploids were obtained in 0.05mg/1 of CB treated fertilized
eggs.
Another chemical 6-Dimethylarninopurine (6-
DMAP) at 50, 100, 150 and 200/lM was tried at various treatment
durations of 5,8 and 10 minutes. High percentage of 66.6 triploids
was obtained from the fertilized eggs treated in 100/lM
concentration of 6-DMAP for 8 minutes.
Among the treatments applied for production of
meiotic triploids, 6-DMAP (IOO/lM) yielded high percentage (66 .6)
of triploids followed by 42.3% by cold (5°C) treatment and 40% in
heat (37°C) and CB (0.05mg/l) treated eggs.
 
Date 2004
 
Type Thesis
NonPeerReviewed
 
Format application/pdf
 
Language en
 
Identifier http://eprints.cmfri.org.in/7251/1/TH-135.pdf
Mallia, Jyothi V (2004) Induction and evaluation of triploidy in Crassostrea madrasensis (Preston)(TH 135). ["eprint_fieldopt_thesis_type_phd" not defined] thesis, Manonmaniam Sundaranar University, Thirunelveli.