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Studies on the use of probiotics in the Larval rearing of the shrimp Penaeus monodon (Fabricius, 1798)

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Relation http://eprints.cmfri.org.in/7512/
 
Title Studies on the use of probiotics in the
Larval rearing of the shrimp Penaeus
monodon (Fabricius, 1798)
 
Creator Anikumari, N P
 
Subject Theses
Crustacean Fisheries
Prawn and Prawn fisheries
 
Description The study regarding 'Studies on the use of probiotics in the larval rearing of the
shrimp Penaeus monodon . was carried out by studying the effect of three probiOlic
organisms. Saccharomyces boulardii. Lactobacillus plal1farulll and Bacillus sublilis. The
parameters monitored were. level of enrichment in larvae and post larvae, survival. weight
gain, effect on other bacterial flora and resistance to a pathogenic Vibrio harveyii infection.
Attempts were made to find out the optimum enrichment duration and maximum enrichment
levels of the above probiotic organisms in Arlemia nauplii and also the difference in
enrichment level between two brands of Arlemia nauplii . The study \\as carried out in larval
as well as in post larval stages. Direct additions of probiOlics were carried out in larval stages
while Saccharomyces boulardii and Bacillus sublilis enriched Arlemia metanauplii \\ere fed
to the larvae in post larval section. Anemia enrichment experiments revealed the maximum
enrichment levels as 3410 for Saccharomyces boulardii. 2093 for Bacillus sublilis and 105
CFUllarva for Laclobacillus plantarum. Enrichment levels also varied between the t\\O
brands. 3410 CFU/nauplii for OSI and 1120 CFU/nauplii for Red Dragon brand in the case of
Saccharomyces boulardii. Significant increase in weight gain and survival rate were noticed
in larvae as well as post larvae in the experiments conducted. Highest enrichment levels were
noticed for mysis Ill. Maximum enrichment levels noticed were 1535 CFUllarva for
Saccharomyces boulardii and 2133 CFUllarva for Bacillus subtilis in the larval stages from
zoea to PL I. while in post larvae it was I CFUllarva for Saccharomyces boulardii and 66
CFUllarva for Bacillus sub/ilis. Laclobacills planlarum could not be detected during the
enrichment study. Addition of Saccharomyces bOl/lardii as \\ell as Bacillus sub/ilis
significantly reduced Vibrio counts during mysis stage as well as during post larval stages.
Effect on sea water agar counts were not significant during larval stages while in post larvae
sea water agar counts were decreased with the application of Saccharomyces boulardii and
Bacillus subtilis. Enrichment of Lactobacillus plan/anlln did not produce any significant
effect on other bacterial flora. Addition of Saccharomyces boulardii and Bacillus sublilis
increased the resistance of larvae to pathogenic Vibrio hmwyii infection in larvae as \I ell as
post larvae. About 103 CFU/ml Saccharomyces boulardii broth and 104 to 105 CFU/ml of
Bacillus sublilis cells were necessary to surmount Vibrio han'eyii infection in PL. I. Post
colonization studies after discontinuing the feeding resulted in 98% reduction in
Saccharomyces boulardii counts and 13% reduction in Bacillus sublilis counts after five days
from PL I. In post larvae Bac/lus sub/ilis showed only 7% reduction in five days from PL 21.
Post larvae fed with live enriched nauplii performed better than those fed with dead enriched
nauplii. for the parameters studied. From the above study it is evident that Saccharomyces
boulardii and Bacilllls sub/lis can be used as effective probiotic species for shrimp
larviculture while Laclobacillusplantarum is not a suitable species.
 
Date 2004
 
Type Thesis
NonPeerReviewed
 
Format application/pdf
 
Language en
 
Identifier http://eprints.cmfri.org.in/7512/1/TH-138.pdf
Anikumari, N P (2004) Studies on the use of probiotics in the Larval rearing of the shrimp Penaeus monodon (Fabricius, 1798). ["eprint_fieldopt_thesis_type_phd" not defined] thesis, Central Institute of Fisheries Education, Mumbai.