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Studies on some aspects of the Reproductive physiology of Metapenaeus monoceros (Fabricius)

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Relation http://eprints.cmfri.org.in/7593/
 
Title Studies on some aspects of the
Reproductive physiology of
Metapenaeus monoceros (Fabricius)
 
Creator Abraham, Joice
 
Subject Fish physiology
Theses
Prawn and Prawn fisheries
 
Description Metapenaeus monoceros (Fabricius), also known as 'Speckled shrimp', is
one of the commercially important species contributing substantially to the shrimp
fisheries of India and is a potential candidate species for diversification of shrimp
culture. The processes of oogenesis and spermatogenesis of the species were
studied using light and electron microscopy. Biochemical changes taking place in
ovary, hepatopancreas, haemolymph and muscle tissue were analysed and the
yolk protein vitellin was characterized. The five stages of ovarian development
based on gross morphology were related to those based on cytological changes.
The mean ova diameter increased from 35.68 ~m in immature stage to 230.56
~m in fully mature stage. Cortical bodies, characteristic of fully mature ova of
most penaeids were absent in M.monoceros. Ultrastructure of oocytes at various
developmental stages showed that the mode of synthesis of yolk is mostly
autosynthetic with the cytoplasm abundant in mitochondria, free ribosomes,
rough endoplasmic retiulum and golgi bodies. The process of spermatophore
formation which took place with the help of the secretory activity of columnar
epithelial cells lining the vas deferens was demonstrated. Secretions of the
proximal vas deferens aided in packing the sperm mass and those of the
Spermatophoric duct of median vas deferens formed the primary spermatophoric
layer. The accessory duct secreted accessory structures associated with the
spermatophore while secretions of the epithelial cells lining the distal vas
deferens formed the secondary spermatophoric layer. Adhesive globules were
formed in the terminal ampoule, where the final moulding of spermatophore took
place. Spermatozoa in this species were unistellate. Variations in the biochemical
constituents in different tissues showed cyclic changes in their concentration
during the various maturity stages except in the muscle tissue. Mobilization of
proteins from the hepatopancreas to ovary was minimal. Haemolymph proteins
showed a declining trend with vitellogenesis, which is due to the transfer to
ovary. A partial mobilization of lipids from the hepatopancreas to the ovary was
noticed. Native polyacrylamide gel electrophoresis of ovarian tissue revealed that
vitellin showed a steady increase in the level of expression with progression in
maturity. Native vitellin in this species has a molecular weight of 326 kD and is a
lipo-glycoprotein bound to calcium.
 
Date 2005
 
Type Thesis
NonPeerReviewed
 
Format application/pdf
 
Language en
 
Identifier http://eprints.cmfri.org.in/7593/1/TH-146.pdf
Abraham, Joice (2005) Studies on some aspects of the Reproductive physiology of Metapenaeus monoceros (Fabricius). ["eprint_fieldopt_thesis_type_phd" not defined] thesis, Central Institute of Fisheries Education, Mumbai.