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Some studies on reproductive Physiology of the female grouper, Epinephelus diacanthus (Valenciennes)

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Relation http://eprints.cmfri.org.in/7606/
 
Title Some studies on reproductive
Physiology of the female grouper,
Epinephelus diacanthus
(Valenciennes)
 
Creator Rao, A Chandrasekhara
 
Subject Fish physiology
Theses
 
Description Groupers are popular food fish farmed in South East Asia and have potential to become
an important cultivable species owing to their fast growth, efficient feed conversion and
high market price. Commercially important species contributing to the grouper fishery
mainly includes Epinephelus diacanthus E. bleekeri, E. chlorostigma, E. malabaricus
and E. tauvina. A major bottleneck in the large scale onshore cu~ure activities of
groupers in our country is the lack of adequate number of hatchery produced seed of
groupers since wild seed availability is highly fluctuating and unpredictable. In order to
master controlled breeding and achieve mass scale production of seed of any cu~ivable
fin fish species, an in depth understanding of the complex physiological changes that
occur at intracellular and intercellular level in the fish body during the process of
reproduction is inev~able . The gonads with the onset of maturation show a series of
developmental changes, which are closely accompanied by the cellular, biochemical,
and molecular changes. It is against this background that the present study has been
taken up on the reproductive physiology of female grouper E. diacanthus which sustain
one of the major demersal fisheries of our country. The study was carried out from
collections of the fish onboard FSI vessel during the cruises off Quilon and off Ratnagiri
region. In this study, the morphology of the female reproductive system has been
described in detail followed by the detailed classification of maturity stages from stage I
to stage III. Data on GSI (Gonadosomatic Index), HSI (Hepatosomatic Index), CF
(Condition Factor), fecund~ etc. have also been obtained and statistically interpreted.
The detailed histology of oocytes and hepatocytes has been described through
microscopic and ultra structural studies to understand inter cellular and intra cellular
changes happening during oogenesis. In the ovary the occurrence of sex inversion has
also been described. Through non denatured Native and denatured SOS-PAGE, the
characterization of vitellogen in in ovarian homogenate and blood serum has been
clearly elaborated. The molecular weight determination of viitellogenin protein fractions
was worked out in the SOS-PAGE. To gather support data on the biochemical changes
occurring during the maturation seven major parameters from four different tissues have
also been studied. The information generated in this study along the above said aspects
is expected to form a basis for initiating further studies on larger groupers.
 
Date 2007
 
Type Thesis
NonPeerReviewed
 
Format application/pdf
 
Language en
 
Identifier http://eprints.cmfri.org.in/7606/1/TH-149.pdf
Rao, A Chandrasekhara (2007) Some studies on reproductive Physiology of the female grouper, Epinephelus diacanthus (Valenciennes). ["eprint_fieldopt_thesis_type_phd" not defined] thesis, Central Institute of Fisheries Education, Mumbai.