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Histomorphological characterization of immune system in greasy grouper, Epinephelus tauvina (Forsskal, 1775)

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Relation http://eprints.cmfri.org.in/7665/
 
Title Histomorphological characterization
of immune system in greasy grouper,
Epinephelus tauvina (Forsskal, 1775)
 
Creator Patra, Sushanta Kumar
 
Subject Fish Biotechnology
Theses
Groupers
 
Description The greasy grouper, Epinephe/us tauvina is one of the major candidate species
cultured in saltwater ponds and cages all over the world. There is scanty information on
immunology of the species and thus an attempt was made to characterize the immune
system of the species. The peripheral leucocytes of the species were characterized by
means of routine haematological techniques, cytochemical methods and transmission
electron microscopy. The haemopoietic organs like thymus, kidney and spleen of the
species were studied by routine histology and electron microscopy. The mean total
erythrocyte and leucocyte counts were 4.42 x 10 6/mm3 ± 0.69 and 72.75 x 10 3/mm3 ±
0.85 respectively. The mean serum total protein, albumin, globulin and albumin/globulin
ratio were 2.04 g % ± 0.23, 0.05 g%± 0.01 , 1.95 g %± 0.21 and 0.02 ± O. 002
respectively. The electrophoretic separation of sera of the greasy grouper yielded 8 to
10 bands of which 4 to 5 bands were prominent. The differential counts of the blood
contained three types of leucocytes namely granulocytes, lymphocytes and monocytes.
The granulocytes were positive for peroxidase, sudan black B, PAS and mildly positive
for acid phosphatase. The ultrastructure of granulocytes indicated two types of cells and
the majority of them had homogeneous electron dense cytoplasmic granules while the
other had crystalloid granules. The mean lymphocyte count was 61 .83 % ± 2.67 and two
types of lymphocytes, large and small lymphocytes, were noticed. The fine structures of
the lymphocytes consisted of nucleus with euchromatin and heterochromatin and the
cytoplasm contained mitochondria, numerous polyribosomes and stacks of rough
endoplasmic reticulum. The monocytes formed 6.33 %± 1.30 of total leucocytes which
were positive for PAS and acid phosphatase. The monocytes had foamy cytoplasm with
peripherally located nucleus. The cytoplasm of monocytes contained Iysosomes,
mitochondria and rough endoplasmic reticulum. The thymus was surrounded by
collagenous capsule and divided into several lobules by trabeculae. The lobules were
supported by the reticular connective tissue, in which epithelial cells and Iymphoblasts
were seen. The ultrastructure of the thymus revealed capillaries lined by endothelial
cells having tight junction and the lumen of the capillaries were filled with blood cells.
The spleen was surrounded by the capsule and a random distribution of the
mesenchymal and parenchymal tissue were noticed. The capillaries were enclosed with
reticular-collagen fibre sheath and filled with macrophages and Iymphoblasts. The
ultrastructural studies revealed the phenomenon of haemopoieosis in spleen. The
kidneys showed the presence of haemopoietic tissue in both histological and
ultrastructural studies. Inflammatory studies yielded biphasic exudation of neutrophils
and Iymphoblasts at the site of injection. In vivo phagocytosis studies on Indian ink
revealed carbon filled phagocytes in the peritoneum at 24 h post injection and the
phagocytic index was 30.15% ± 0.46. The skin sensitivity test using PHA showed
thickening reaction which prolonged more than 72 h. The rosette forming cells with
rabbit erythrocytes were 39.67 % ± 0.71 .
 
Date 2006
 
Type Thesis
NonPeerReviewed
 
Format application/pdf
 
Language en
 
Identifier http://eprints.cmfri.org.in/7665/1/TH-152.pdf
Patra, Sushanta Kumar (2006) Histomorphological characterization of immune system in greasy grouper, Epinephelus tauvina (Forsskal, 1775). ["eprint_fieldopt_thesis_type_phd" not defined] thesis, Central Institute of Fisheries Education, Mumbai.