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Pathobiological studies of Vibrio alginolyticus biofilm on the defense functions and disease resistance in Giant tiger shrimp (Penaeus monodon)

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Relation http://eprints.cmfri.org.in/7667/
 
Title Pathobiological studies of
Vibrio alginolyticus biofilm on the defense
functions and disease resistance in
Giant tiger shrimp (Penaeus monodon)
 
Creator Sharma, S R Krupesha
 
Subject Theses
Fish Diseases
Prawn and Prawn fisheries
 
Description The present study was undertaken to develop and evaluate the
biofIlm of Vibrio alginolyticus for oral immunostimulation in tiger shrimp,
Penaeus monodon.
Development of biofIlm of V. alginolyticus was standardized and
maximum bioftlm formation was achieved with 0.15 per cent TSB
supplemented with 2 per cent NaCI on 0 .3 per cent chitin flakes with
incubation for 3 d. The biofllm cell population was inversely related to
the planktonic cell population during the culture. The biofllm cells could
be inactivated at 800C and 10 per cent formalin while free cells at 600C
and 0.5 per cent formalin suggesting the resistant nature of bioftlm to
action by heat and chemicals.
In SDS-PAGE, biofllm cells showed an increased expression of 3
new proteirts and repression of 4 proteins compared to free cells.
Elevated values of the immune parameters with respect to total
haemocyte count, phenol oxidase activity, total haemolymph protein and
antibacterial activity were observed in shrimps exposed to 108 , 109 and
1010 cfu/g shrimp/ day bioftlm cells when compared to control shrimps.
The values were highest in 109 and 1010 cfu groups with no significant
difference between them. It was observed that 109 cfu/ g shrimp/day for
14 days was the optimum dose and time required to activate the immune
system of juvenile P. monodon.
 
Date 2008
 
Type Thesis
NonPeerReviewed
 
Format application/pdf
 
Language en
 
Identifier http://eprints.cmfri.org.in/7667/1/TH-153.pdf
Sharma, S R Krupesha (2008) Pathobiological studies of Vibrio alginolyticus biofilm on the defense functions and disease resistance in Giant tiger shrimp (Penaeus monodon). ["eprint_fieldopt_thesis_type_phd" not defined] thesis, Karnataka Veterinary, Animal and Fisheries Sciences, Bidar.