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Studies on the defence and stress related factors of Oyster and Mussels of mariculture importance

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Relation http://eprints.cmfri.org.in/10022/
 
Title Studies on the
defence and stress related factors of Oyster and Mussels
of mariculture importance
 
Creator Paulton, M P
 
Subject Fish physiology
Theses
Bivalves
 
Description Marine bivalves are regularly exposed to biotic and abiotic stressors throughout
the life in their natural habitat. The molecular mechanism with which the intertidal
oysters and mussels manage such situations are mediated through defence and stress
related proteins such as heat shock proteins and antioxidant enzymes. The present study
was aimed at , molecular identification, characterization and expression analysis of the genes
of selected heat shock proteins and antioxidant enzymes in the Indian edible oyster
Crassostrea madrasensis and tropical brown mussel Perna indica. Molecular identification
and complete characterization of the heat shock protein70 (HSP70) gene and the antioxidant
enzyme superoxide dismutase (Cu/Zn SOD) gene of both the species were successfully
carried out. Amino acid sequences were deduced from the full length coding sequences and
used for the identification of the characteristic domains, homology search, and phylogenetic
analysis etc.. The study was also extended to the partial characterization of the gene coding
for heat shock protein90 (HSP90) as well as other antioxidant enzymes such as glutathione
peroxidase (GPX) and catalase (CAT), and an important peptidase gene Leucine
aminopeptidase (LAP) with vital role in protein turnover and salinity tolerance. The sequences
were deposited in NCBI GenBank.
The temperature tolerance limits such as sub lethal temperature (SLT) and lethal
temperature (LT) in both the species were determined as a pre-mediated step for the study
of gene expression induced by exposure to sub lethal thermal shock. The SLT and LT limits
of the candidate species living in the tropical waters were revealed to be higher than their
counterparts in temperate waters. The Indian edible oyster was found to be the most thermo
tolerant among the various oyster species.
A semi quantitative PCR approach was adopted to study the relative gene expressions
in animals exposed to sub lethal temperature as well as in control animals. The genes like
HSP70 and SOD were found to be up regulated on exposure to sub lethal temperature. The
other genes studied were also found to be up regulated in general with slight variations in
the expressions. All the gene expressions were found to be statistically significant (P
 
Date 2012
 
Type Thesis
NonPeerReviewed
 
Format text
 
Language en
 
Identifier http://eprints.cmfri.org.in/10022/1/Ph.D_Thesis_of_Dr._M.P.Paulton.pdf
Paulton, M P (2012) Studies on the defence and stress related factors of Oyster and Mussels of mariculture importance. Doctoral thesis, Mangalore University, Mangalore.