Record Details

PCR-based sex determination of cetaceans and dugong from the Indian seas

CMFRI Repository

View Archive Info
 
 
Field Value
 
Relation http://eprints.cmfri.org.in/2/
http://www.ias.ac.in/currsci/jun102008/1513.pdf
 
Title PCR-based sex determination of
cetaceans and dugong from the
Indian seas
 
Creator Jayasankar, P
Anoop, B
Rajagopalan, M
 
Subject Fish Genetics
 
Description A sex-determination technique based on PCR amplifi- cation of genomic DNA extracted from the skin tissue has been standardized in cetaceans and dugong sam-pled from the Indian seas. A Y-chromosome-specific region (SRY or Sex-determining Y-chromosome gene) of 210–224 bp size in the genome has been amplified (only in males) using specific PCR primers. A fragment of the ZFX/ZFY (zinc finger protein genes located both on the X and Y chromosomes respectively) re-gion in the size range 442–445 bp is also amplified (in both sexes) using another pair of primers simultaneously as positive controls for confirmation of sex. Molecular sexing was standardized in spinner dolphin (Stenella longirostris), bridled dolphin (Stenella attenuata), bottlenose dolphin (Tursiops aduncus), Indo-Pacific humpbacked dolphin (Sousa chinensis), Risso’s dolphin (Grampus griseus), finless porpoise (Neopho-caena phocaenoides), sperm whale (Physeter macro-cephalus), blue whale (Balaenoptera musculus), Bryde’s whale (Balaenoptera edeni) and dugong (Dugong du-gon), which are all vulnerable/endangered species pro- tected under the Indian Wildlife Act.
 
Publisher Current Science Association
 
Date 2008
 
Type Article
PeerReviewed
 
Format application/pdf
 
Language en
 
Identifier http://eprints.cmfri.org.in/2/1/1513.pdf
Jayasankar, P and Anoop, B and Rajagopalan, M (2008) PCR-based sex determination of cetaceans and dugong from the Indian seas. Current Science, 94 (11). pp. 1513-1516.