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A cell culture system developed from heart tissue of the greasy grouper, Epinephelus tauvina (Forsskal 1775) by enzymatic dissociation

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Relation http://eprints.cmfri.org.in/110/
 
Title A cell culture system developed from heart tissue of the greasy grouper,
Epinephelus tauvina (Forsskal 1775) by enzymatic dissociation
 
Creator Sobhana, K S
George, K C
Venkat Ravi, G
Ittoop, Gijo
Paulraj, R
 
Subject Groupers
 
Description Heart tissue samples aseptically excised from healthy juveniles of the grouper, Epinephelus tauvina were subjected to enzymatic
dissociation using 0.25 % trypsin solution, under aseptic conditions. The resultant cells were suspended in Leibovitz' L-15
medium supplemented with 20 % fetal bovine serum. The cells were subsequently seeded into 25 cm2 tissue culture flasks at
a density of 106 ml-1 and incubated at 28 ± 2°C. Cells showed spreading and attachment to the culture flasks within 24 h and
formed monolayers comprising of epithelioid as well as fibroblast-like cells within 20 days. A confluent monolayer filling
the flask surface comprising predominantly fibroblastic cells was formed within 30 to 35 days. The cell monolayer was
harvested for passage by trypsinisation, which formed successful monolayers in subsequent subcultures.
 
Publisher CMFRI
 
Date 2008
 
Type Article
PeerReviewed
 
Format application/pdf
 
Language en
 
Identifier http://eprints.cmfri.org.in/110/1/ijf55%283%29Shobana.pdf
Sobhana, K S and George, K C and Venkat Ravi, G and Ittoop, Gijo and Paulraj, R (2008) A cell culture system developed from heart tissue of the greasy grouper, Epinephelus tauvina (Forsskal 1775) by enzymatic dissociation. Indian Journal of Fisheries, 55 (3). pp. 251-256.