Record Details

Maintenance of haemocytes of edible oyster, Crassostrea madrasensis (Preston) in artificial media

CMFRI Repository

View Archive Info
 
 
Field Value
 
Relation http://eprints.cmfri.org.in/131/
 
Title Maintenance of haemocytes of edible oyster,
Crassostrea madrasensis (Preston) in artificial
media
 
Creator Ittoop, Gijo
George, K C
Sanil, N K
Sobhana, K S
Nisha, P C
 
Subject Molluscan Fisheries
Fish Biotechnology
 
Description The haemocytes of Crassostrea madrasensis were maintained in Hank's balanced
salt solution (HBSS), HBSS with 10% foetal calf serum (FCS), tissue culture
media M 199, and M 199 with 10% FCS. Total counts of viable haemocytes were
taken at 0 hr, 24 hrs, 48 hrs, and 72 hrs. HBSS and HBSS with 10% FCS were
found to maintain cell viability upto 72 hrs without much reduction in the count
of viable cells. In M199 and M199 with 10% FCS, it was found that a substantial
increase of small hyalinocytes occurred at 24 hrs and 48 hrs. This was followed by
a steep fall in the number of viable cells at 72 hrs in both the media. The HBSS,
HBSS with 10% FCS, M199 and M199 with 10% FCS were found to be good for
maintaining the cells in viable state. However, only M 199 and M 199 with 10%
FCS indicated multiplication of haemocytes.
 
Publisher CMFRI/ICAR
 
Date 2001
 
Type Article
PeerReviewed
 
Format application/pdf
 
Language en
 
Identifier http://eprints.cmfri.org.in/131/1/Article_11.pdf
Ittoop, Gijo and George, K C and Sanil, N K and Sobhana, K S and Nisha, P C (2001) Maintenance of haemocytes of edible oyster, Crassostrea madrasensis (Preston) in artificial media. Indian Journal of Fisheries, 48 (2). pp. 177-182.