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Characterisation of vitellogenin and vitellin of Fenneropenaeus merguiensis (De Man)

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Relation http://eprints.cmfri.org.in/158/
 
Title Characterisation of vitellogenin and vitellin
of Fenneropenaeus merguiensis (De Man)
 
Creator Zacharia, Sherly
Kakati, V S
 
Subject Crustacean Fisheries
 
Description Purification and characterisation of vitellogenin and vitellin from haemolymph
and mature ovaries respectively of Fenneropenaeus merguiensis were conducted
using ammonium sulphate precipitation technique. The vitellogenin level in
the haemolymph was low in the early stages of development, but increased
significantly in the late maturing stages (Stage III) and fell abruptly in spent
females. Purified vitellogenin had an approximate molecular weight of 350kDa
as determined by PAGE and was characterised as lipoglycoprotein with calcium
affinity on the basis of staining data. Two forms of purified vitellin (Vn1 and
Vn2) of molecular weights 550 and 300kDa were also isolated. Vn1 was
characterised as a lipo-glyco-calcium complex while Vn2 was glycoprotein in
nature based on staining data. SDS PAGE analysis revealed 6 subunits in
purified vitellogenin and 5 subunits in vitellin.
 
Publisher CMFRI
 
Date 2004
 
Type Article
PeerReviewed
 
Format application/pdf
 
Language en
 
Identifier http://eprints.cmfri.org.in/158/1/IJF_51_no.3_%2C2004_-_1.pdf
Zacharia, Sherly and Kakati, V S (2004) Characterisation of vitellogenin and vitellin of Fenneropenaeus merguiensis (De Man). Indian Journal of Fisheries, 51 (3). pp. 255-263.