ICAR Research Data Repository for Knowledge Management
Cryopreservation of spermatophores of the marine shrimp, Penaeus indicus H. Milne Edwards
CMFRI Repository
View Archive Info| Field | Value | |
| Relation |
http://eprints.cmfri.org.in/160/
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| Title |
Cryopreservation of spermatophores of the marine shrimp, Penaeus indicus H. Milne Edwards |
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| Creator |
Diwan, A D
Joseph, Shoji |
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| Subject |
Fish physiology
Prawn and Prawn fisheries |
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| Description |
Attempts on cryopreservation of spermatozoa have been made in penaeid shrimp, Penaeus indicus. Viable spermatozoa could be preserved successfully for a period of 60 days at -35°C and -196°C. Among several cryoprotectants used, a combination of DMSO (5 %) and glycerol (5 %), DMSO (5 %) and trehalose (0.25M) showed best viability rate (75 to 80 %) in freeze, thawed spermatozoa. Activational changes in spermatozoa during acrosome reaction after induction with egg water have also been recorded. The importance of cryogenic storage of spermatozoa is discussed. |
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| Publisher |
CMFRI/ICAR
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| Date |
1999
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| Type |
Article
PeerReviewed |
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| Format |
application/pdf
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| Language |
en
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| Identifier |
http://eprints.cmfri.org.in/160/1/Article_08.pdf
Diwan, A D and Joseph, Shoji (1999) Cryopreservation of spermatophores of the marine shrimp, Penaeus indicus H. Milne Edwards. Indian Journal of Fisheries, 46 (2). pp. 159-166. |
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