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Studies on cryogenic preservation of sperm of certain cultivable marine fishes

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Relation http://eprints.cmfri.org.in/186/
 
Title Studies on cryogenic preservation of sperm of
certain cultivable marine fishes
 
Creator Diwan, A D
Nandakumar, A
 
Subject Fish physiology
 
Description Significant success has been achieved in long-term cryopreservation of sperm
at-196°C motility of certain marine fishes like Liza parsia, Sillago sihama,
Mugil cephalus and Gerres oyena. In case of L. parsia sperm motility could
be preserved successfully for more than 240 days using suitable cryoprotectant.
In case of S. sihama and M. cephalus viable sperm could be cryopreserved
for 1 month only, whereas in the case of G. oyena viable sperm could be
preserved for a period of 3 months. While attempting on short-term
preservation of sperm in L. parsia at -10°C, viability could be maintained
for 24 hrs and beyond this at 48 hrs there was total loss of motility. Analysis
of biochemical constituents of seminal plasma in case of L. parsia was carried
out to know the possible causative factors for the loss of motility of sperm and
it was found that the glucose and protein levels decreased significantly in the
seminal fluid cryopreserved for 48 hrs whereas lipid content increased.
Similarly there was drastic reduction in the levels of Na+ and K+ ions in the
seminal fluid cryopreserved for 24 hrs.
 
Publisher CMFRI/ICAR
 
Date 1998
 
Type Article
PeerReviewed
 
Format application/pdf
 
Language en
 
Identifier http://eprints.cmfri.org.in/186/1/Article_05.pdf
Diwan, A D and Nandakumar, A (1998) Studies on cryogenic preservation of sperm of certain cultivable marine fishes. Indian Journal of Fisheries, 45 (4). pp. 387-397.