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Characterization of phenoloxidase of Penaeus indicus

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Relation http://eprints.cmfri.org.in/498/
 
Title Characterization of phenoloxidase of Penaeus indicus
 
Creator Anil Kumar, P
Sridhar, N
 
Subject Fish Biotechnology
Prawn and Prawn fisheries
 
Description The enzyme phenoloxidase from haemolymph of Penaeus indicus was partially purified us-
ing ammonium sulphate fractionation technique. An overall recovery of 52% with a 5-fold
purification was achieved. Dialysis reduced the enzyme activity indicating the presence of
dialysable cofactors. The enzyme had high afinity for the biphenolic substrate adrenalin fol-
lowed by dopamine, catechol and dopa. The rate of the reaction was linear up to 3 min when
adrenalin was used as the substrate. The enzyme had an optimum tem erature of 50°C but lost 60% of its activity when heated to 70°C for 10 min. The Krn and t"max of the enzyme with
adrenalin as the substrate was 0.122 mM and 0.588 OD unitslmg protein a min respectively.
The enzyme was inhibited by the substrate adrenalin at a concentration of IS mM and by
cupric salts. Magnesium and calcium stimulated the enzyme activity. EDTA and mercaptoethanol
strongly inhibited the enzyme.
 
Publisher CMFRI/ICAR
 
Date 1993
 
Type Article
PeerReviewed
 
Format application/pdf
 
Language en
 
Identifier http://eprints.cmfri.org.in/498/1/Vol_40_No_4-5%3B251-255.pdf
Anil Kumar, P and Sridhar, N (1993) Characterization of phenoloxidase of Penaeus indicus. Indian Journal of Fisheries, 40 (4). pp. 251-255.