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Genomic dissection and expression analysis of stress-responsive genes in C4 panicoid models, Setaria italica and Setaria viridis

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Title Genomic dissection and expression analysis of stress-responsive genes in C4 panicoid models, Setaria italica and Setaria viridis
 
Creator Muthamilarasan, Mehanathan
Singh, Roshan Kumar
Suresh, Bonthala Venkata
Rana, Sumi
Dulani, Priya
Prasad, Manoj
 
Subject C4photosynthesis
Expression profiling
Comparative mapping
Evolution
Foxtail millet (Setaria italica)
Green foxtail (Setaria viridis)
 
Description Accepted date: 11 May 2020
The study reports the identification and expression profiling of five major classes of C4 pathway-specific genes, namely, carbonic anhydrase (CaH), phosphoenolpyruvate carboxylase (PEPC), pyruvate orthophosphate dikinase (PPDK), NADP-dependent malate dehydrogenase (MDH) and NADP-dependent malic enzyme (NADP-ME), in the model species, Setaria italica and Setaria viridis. A total of 42 and 41 genes were identified in S. italica and S. viridis, respectively. Further analysis revealed that segmental and tandem duplications have contributed to the expansion of these gene families. RNA-Seq derived expression profiles of the gene family members showed their differential expression pattern in tissues and dehydration stress. Comparative genome mapping and Ks dating provided insights into their duplication and divergence in the course of evolution. Expression profiling of candidate genes in contrasting S. italica cultivars subjected to abiotic stresses and hormone treatments showed distinct stress-specific upregulation of SiαCaH1, SiβCaH5, SiPEPC2, SiPPDK2, SiMDH8, and SiNADP-ME5 in the tolerant cultivar. Overexpression of SiNADP-ME5 in heterologous yeast system enabled the transgenic cells to survive and grow in dehydration stress conditions, which highlights the putative role of SiNADP-ME5 in conferring tolerance to dehydration stress. Altogether, the study highlights key genes that could be potential candidates for elucidating their functional roles in abiotic stress response.
This study was funded by the Core Grant of National Institute of
Plant Genome Research, New Delhi, India and the DST INSPIRE Faculty
Grant of Department of Science & Technology (DST), Ministry of
Science & Technology, Government of India (File No. DST/INSPIRE/
04/2016/002341). M.M. acknowledges DST INSPIRE Faculty Award
from Department of Science & Technology, Ministry of Science &
Technology, Government of India. R.K.S. is thankful to Council of
Scientific & Industrial Research, Ministry of Science & Technology,
Government of India for the Research Fellowship. The authors are
thankful to DBT-eLibrary Consortium (DeLCON) for providing access to
e-resources.
 
Date 2020-05-27T06:24:29Z
2020-05-27T06:24:29Z
2020
 
Type Article
 
Identifier Journal of Biotechnology, 318: 57-67
0168-1656
https://doi.org/10.1016/j.jbiotec.2020.05.007
https://www.sciencedirect.com/science/article/pii/S0168165620301279
http://223.31.159.10:8080/jspui/handle/123456789/1064
 
Language en_US
 
Format application/pdf
 
Publisher Elsevier B.V.