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In vitro culture of mantle tissue of the abalone Haliotis varia Linnaeus

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Relation http://eprints.cmfri.org.in/1307/
 
Title In vitro culture of mantle tissue of the abalone Haliotis varia Linnaeus
 
Creator Suja, C P
Dharmaraj, S
 
Subject Gastropods
 
Description The study is aimed at developing a technology for the production of in vitro pearl through tissue culture of mantle of the abalone, Haliotis
varia Linnaeus, as the production of free and spherical pearls in vivo is rather difficult in abalones. In the basic study, the cell yield was
intensified from the explant after 24 h incubation. Among the cells liberated, the granulocytes were dominant over hyalinocytes. The size
of granulocytes ranged from 3 to 16�m and of hyalinocytes from 13 to 18�m. Fibroblast-like cells appeared in cultures after day 2. Both
granulocytes and hyalinocytes developed pseudopodial-like extensions in all directions and formed organic matrix. Granulocytes contained
granules in the cytoplasm. Specific granules were responsible for nucleation of crystals. Some crystals exhibited green colour resembling
mother of pearl of abalone. scanning electron microscope (SEM) study revealed the oolitic amorphous state and rhombohedral state of
crystals. Its analysis through energy dispersive X-ray microanalyzer (EDS) indicated the presence of calcium. The rhombohedral crystals
under polarized light showed its high birefringence (0.18) and uniaxial optically negative calcite nature with high content of calcium. A mean
survival of cells was found to be 102 days in T 25 flasks and 32 days in petri dishes. Growth of cells was studied. Thirty percent of cultures
were found to have contaminated during the study. The study provides basic knowledge in the development of a technology for in vitro pearl
production.
 
Publisher Elsevier
 
Date 2005
 
Type Article
PeerReviewed
 
Format application/pdf
 
Language en
 
Identifier http://eprints.cmfri.org.in/1307/1/tissue_and_cellvol37p1-10.pdf
Suja, C P and Dharmaraj, S (2005) In vitro culture of mantle tissue of the abalone Haliotis varia Linnaeus. Tissue and Cell, 37. pp. 1-10.