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Application of molecular tools in support of deployment of Theileria parva live vaccines

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Title Application of molecular tools in support of deployment of Theileria parva live vaccines
 
Creator Bishop, Richard P.
Nene, Vishvanath
Spooner, P.R.
Mbogo, S.
Kariuki, D.
Payne, R.
Morzaria, S.P.
 
Subject THELERIA PARVA
LIVE VACCINES
IMMUNOLOGICAL TECHNIQUES
QUALITY CONTROLS
DNA
MARKERS
PCR
POPULATION GENETICS
 
Description The primary goals of applying molecular tools, developed at ILRI in conjunction with the use of infection and treatment immunisation are quality control of vaccines; monitoring of possible vaccine breakthrough and breakdown; and assessment of the impact of introducing new parasite types on long-term efficacy of the vaccine. There are two main categories of tools available for use; serological such as schizont - specific monoclonal antibodies and DNA-based. This paper outlines serological techniques; and DNA-based techniques including southern blotting, polymerase chain reaction (PCR) and single strand conformation polymorphism (SSCP). It discusses quality control and markers for vaccine stocks; investigating possible vaccine breakdown and breakthrough; molecular epidemiology of ECF which deals with role of other theileria species, genetic variation between T. parva isolates and population genetics.
 
Date 2010-12-09T11:24:22Z
2010-12-09T11:24:22Z
1999
 
Type Conference Paper
 
Identifier ISBN 92-9146-055-9
https://hdl.handle.net/10568/2865
 
Language en
 
Publisher ILRI