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Halocatena pleomorpha Gen. Nov. Sp. Nov., an Extremely Halophilic Archaeon of Family Halobacteriaceae Isolated From Saltpan Soil.

DIR@IMTECH: CSIR-Institute of Microbial Technology

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Title Halocatena pleomorpha Gen. Nov. Sp. Nov., an Extremely Halophilic Archaeon of Family Halobacteriaceae Isolated From Saltpan Soil.
 
Creator Verma, Ashish
Pal, Yash
Kumar, Pravin
Krishnamurthi, Srinivasan
 
Subject QR Microbiology
 
Description A novel archaeal strain designated as SPP-AMP-1T was isolated from saltpan soil, using the serial dilution method on a halophilic archaeal medium supplemented with ampicillin. Cells were both rod-shaped and pleomorphic in nature, non-motile, unable to produce acid from a variety of sugars or grow anaerobically with different substrates (l-arginine) and electron acceptors (DMSO, nitrate). Optimal growth was observed at 42 °C, 3.4-4.2 M NaCl and pH 7.2. Cells did not lyse in distilled water and grew in the absence of Mg2+ ions. Phylogenetic analysis based on the sequences of 16S rRNA gene, amino acid sequence of β'-subunit of RNA polymerase and 400 conserved proteins retrieved from the whole genome assemblies showed that strain SPP-AMP-1T was distantly related to any existing genera within the family Halobacteriaceae. MK-8 was the only quinone detected. Polar lipid analysis showed a unique combination of diethyl derivatives of phosphatidylglycerol, phosphatidylglycerol phosphate methyl ester, glycosyl-mannosyl-glucosyl diether and sulphated glycosyl-mannosyl-glucosyl diether as the major lipids. The G+C content of genomic DNA is 57.7 mol%. The phenotypic, phylogenetic and genomic data supported the concept of the novel genus status of strain SPP-AMP-1T in the family Halobacteriaceae for which the name Halocatena pleomorpha gen. nov., sp. nov., is proposed; the type strain is SPP-AMP-1T (=JCM 31368T=KCTC 4276T=MTCC 12579T).
 
Publisher International Union of Microbiological Societies
 
Date 2020-05-18
 
Type Article
PeerReviewed
 
Relation https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.004222#tab2
http://crdd.osdd.net/open/2575/
 
Identifier Verma, Ashish and Pal, Yash and Kumar, Pravin and Krishnamurthi, Srinivasan (2020) Halocatena pleomorpha Gen. Nov. Sp. Nov., an Extremely Halophilic Archaeon of Family Halobacteriaceae Isolated From Saltpan Soil. International journal of systematic and evolutionary microbiology. ISSN 1466-5034