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Ultrastructural changes in the spermatozoa of the goldspot mullet Liza parsia (Hamilton-Buchanan) in different diluents during cryopreservation

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Relation http://eprints.cmfri.org.in/2003/
 
Title Ultrastructural changes in the spermatozoa of the goldspot mullet Liza parsia (Hamilton-Buchanan) in different diluents during cryopreservation
 
Creator Sukumaran, Sandhya
Noble, D
Gopalakrishnan, A
Sanil, N K
 
Subject Mullet
 
Description The milt of goldspot mullet, Liza parsia (Hamilton-Buchanan) was diluted with four extenders containing 10% DMSO. Samples were collected at four critical steps of cryopreservation procedure. Percentage of intact spermatozoa and cryoinjuries at each step was recorded. Extender V2E in seawater base +lo% DMSO appeared to be the best cryodiluent as the sperms exhibited least structural changes during the cryopreservation protocol. Chao's extender+lO% DMSO was the next preferred cryodiluent. The suitability of various extenders that accorded protection to injuries was evaluated on the basis of electron microscopic images.
 
Publisher MBAI
 
Date 2003
 
Type Article
PeerReviewed
 
Format application/pdf
 
Language en
 
Identifier http://eprints.cmfri.org.in/2003/1/Sandhya_47-53.pdf
Sukumaran, Sandhya and Noble, D and Gopalakrishnan, A and Sanil, N K (2003) Ultrastructural changes in the spermatozoa of the goldspot mullet Liza parsia (Hamilton-Buchanan) in different diluents during cryopreservation. Journal of the Marine Biological Association of India, 45 (1). pp. 47-53.