Ultrastructural changes in the spermatozoa of the goldspot mullet Liza parsia (Hamilton-Buchanan) in different diluents during cryopreservation
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Relation |
http://eprints.cmfri.org.in/2003/
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Title |
Ultrastructural changes in the spermatozoa of the goldspot mullet Liza parsia (Hamilton-Buchanan) in different diluents during cryopreservation
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Creator |
Sukumaran, Sandhya
Noble, D Gopalakrishnan, A Sanil, N K |
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Subject |
Mullet
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Description |
The milt of goldspot mullet, Liza parsia (Hamilton-Buchanan) was diluted with four extenders containing 10% DMSO. Samples were collected at four critical steps of cryopreservation procedure. Percentage of intact spermatozoa and cryoinjuries at each step was recorded. Extender V2E in seawater base +lo% DMSO appeared to be the best cryodiluent as the sperms exhibited least structural changes during the cryopreservation protocol. Chao's extender+lO% DMSO was the next preferred cryodiluent. The suitability of various extenders that accorded protection to injuries was evaluated on the basis of electron microscopic images.
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Publisher |
MBAI
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Date |
2003
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Type |
Article
PeerReviewed |
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Format |
application/pdf
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Language |
en
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Identifier |
http://eprints.cmfri.org.in/2003/1/Sandhya_47-53.pdf
Sukumaran, Sandhya and Noble, D and Gopalakrishnan, A and Sanil, N K (2003) Ultrastructural changes in the spermatozoa of the goldspot mullet Liza parsia (Hamilton-Buchanan) in different diluents during cryopreservation. Journal of the Marine Biological Association of India, 45 (1). pp. 47-53. |
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