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Triploidy induction and confirmation in the edible oyster Crassosfrea madrasensis

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Relation http://eprints.cmfri.org.in/2035/
 
Title Triploidy induction and confirmation in the edible oyster Crassosfrea madrasensis
 
Creator Thomas, P C
Mallia, Jyothi V
Muthiah, P
 
Subject Edible oyster
 
Description This paper presents the protocols standardized for producing triploid edible oyster, Crassostrea
madrasensis by retention of I1 polar body (MI1 triploids), using physical (heat and cold shocks) and chemical agents (Cytochalasin B and 6-Dimethylaminopurine), as well as the protocol for preparation of metaphase spreads for cytological confirmation of triploidy and estimation of
its percentage. Among the four inducing agents tried, 6-Dimethylaminopurine yielded highest percentage of triploids (66.6 in day old and 61.82 in "D" stage larvae).
 
Publisher MBAI
 
Date 2004
 
Type Article
PeerReviewed
 
Format application/pdf
 
Language en
 
Identifier http://eprints.cmfri.org.in/2035/1/Thomas_224-228.pdf
Thomas, P C and Mallia, Jyothi V and Muthiah, P (2004) Triploidy induction and confirmation in the edible oyster Crassosfrea madrasensis. Journal of the Marine Biological Association of India, 46 (2). pp. 224-228.