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Sample preparation methods for isolation of Mycobacterium spp. from cultured fish and environmental samples

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Relation http://eprints.cmfri.org.in/2379/
 
Title Sample preparation methods for isolation of
Mycobacterium spp. from cultured fish and
environmental samples
 
Creator Latha, M M
Chandrika, V
 
Subject Mariculture
 
Description Fish mycobacteriosis' is a problem to which more than 150
species offish are susceptible. In order to isolate non-pathogenic and
fish pathogenic m.ycobacterial species from environm.ental
samples, three different methods were evaluated. Shaking and
membrane filtration methods were adopted to retrieve maxim.um acidfast
strains from fish and environmental samples.
Decontamination with 4% sodium hydroxide facilitated the isolation
of acid-fast bacteria in the selective media, killing all the contam.inants
like heterotrophs and saprophytes. Centrifugation procedure at 4000
r.p. mfor 20 minutes eliminated the contaminants from the sample and
allowed only acid-fast bacilli to grow in the selective media.
Centrifugation was carried out twice with distilled water. Peizer
TB and LJ slopes worked well for selective isolation of fish
pathogenic Mycobacteria/rom the centrifugated samples. It is
believed that all the three sample preparation methods will be useful
tool to study the fastidious fish pathogenic Mycobacteria from
environmental samples.
 
Publisher The Marine Biological Association of India
 
Contributor Menon, N G
Pillai, P P
 
Date 2001
 
Type Book Section
PeerReviewed
 
Format application/pdf
 
Language en
 
Identifier http://eprints.cmfri.org.in/2379/1/Article_13.pdf
Latha, M M and Chandrika, V (2001) Sample preparation methods for isolation of Mycobacterium spp. from cultured fish and environmental samples. In: Perspectives in Mariculture. The Marine Biological Association of India, Cochin, pp. 149-162.