| Abstract |
Derivation and use of doubled haploid (DH) lines, compared to conventionally-derived inbred lines, offers several advantages to maize breeding programs. This includes reduced time taken to develop and deploy superior maize varieties, simplified logistics and reduced costs in line development and maintenance. Use of DH lines in conjunction with molecular markers significantly improves genetic gains and breeding efficiency. Production of DH lines in maize involves in vivo induction using maternal haploid inducers, identification of haploids using anthocyanin markers, doubling the chromosomes in haploid seedlings, and production of seed from fertile doubled haploid plants. In vivo haploid induction is achieved by crossing a source population (used as a female parent) from which homozygous DH lines are developed, with a specially developed maize genetic stock called a “haploid inducer” (used as a male parent) with high haploid induction capacity. |
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