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Data on plant growth-promoting actinobacteria on Chickpea seed mineral density: an upcoming complementary tool for sustainable biofortification strategy

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Title Data on plant growth-promoting actinobacteria on Chickpea seed mineral density: an upcoming complementary tool for sustainable biofortification strategy
 
Identifier https://doi.org/10.21421/D2/46KKXE
 
Creator Sathya A
Vijayabharathi Rajendran
Srinivas V
Gopalakrishnan, S
 
Publisher ICRISAT Dataverse
 
Description The data set contains, evaluated to test the 19 potential of plant growth-promoting actinobacteria in increasing seed mineral density of Chickpea under field conditions.The field trial conditions used were as follows. This experiment was carried out during 2013–2014 post-rainy cropping season. The experiment was laid out in a randomized complete block design (RCBD) with three replicates and plot sizes of 4 m x 3 ridges (rows). The 19 test strains of PGP actinobacteria were cultured individually on a starch casein broth at 28°C for 5 days. Seeds of chickpea variety ICCV 2 were treated with PGP. The PGP actinobacteria (1000 ml; 108 CFU per mL) was applied once every 15 days on the soil close to the plant until the flowering stage. The harvested seeds were subjected to auto-claving as it mimics pressure cooking, a common household cooking method. For this, the seeds were soaked in water at 1:10 ratio (seed: water, w/v) for 8 h and the soaking water was decanted. The soaked seeds were auto-claved with freshwater in the ratio of 1:5 for 10 min at 121°C. The auto-claved seeds were drained from excess water and allowed to dry at 45 ± 2 OC. The processed dried seeds along with the raw counterparts of harvested seeds were ground into a fine powder using a laboratory blender and stored in airtight polythene bags at 4 °C until further analysis.





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Subject Agricultural Sciences
Calcium
Copper
Magnesium
Manganese
Zinc
Replication number
Chickpea
 
Date 2012-11-14
 
Contributor Administrator
 
Type Phenotypic evaluation
 
Source ICRISAT