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Data on evaluation of actinomycete isolates obtained from herbal vermi compost for the biological control of fusarium wilt of Chickpea

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Title Data on evaluation of actinomycete isolates obtained from herbal vermi compost for the biological control of fusarium wilt of Chickpea
 
Identifier https://doi.org/10.21421/D2/5FRCLM
 
Creator Gopalakrishnan, S
Suresh Pande
Mamta Sharma
Humayun Pagidi
Keerthi Kiran B
Sandeep D
Sreevidya M
Deepthi K
Rupela Om
 
Publisher ICRISAT Dataverse
 
Description Five most promising FOC antagonistic isolates (CAI-24, CAI-121, CAI-127, KAI-32 and KAI-90) were characterized for the production of siderophore, cellulase, protease, hydrocyanic acid (HCN), indole acetic acid (IAA) and antagonistic potential against Rhizoctonia bataticola, which causes dry root rot in chickpea (three strains viz. RB-6, RB-24 and RB-115) and sorghum (one strain). All of the five FOC antagonistic isolates produced siderophore and HCN, four of them (except KAI-90) produced IAA, KAI-32 and KAI-90 produced cellulase and CAI-24 and CAI-127 produced protease. In the dual-culture assay, three of the isolates, CAI-24, KAI-32 and KAI-90, also inhibited all three strains of R. bataticola in chickpea, while two of them (KAI-32 and KAI-90) inhibited the tested strain in sorghum. When the FOC antagonistic isolates were evaluated further for their antagonistic potential in the greenhouse and wilt sick field conditions on chickpea. The five most potential antagonistic actinomycetes from the in vitro and greenhouse studies were further evaluated individually for their antagonistic potential in Fusarium-infested field at ICRISAT. The field was maintained as wilt sick plot since 1980. Actinomycete isolate was inoculated by four different methods. Thus, the combination of actinomycete isolates x four methods of inoculation constituted 20 independent treatments in addition to one positive control, where no actinomycete was inoculated. Each treatment was replicated three times in randomized complete block design (RCBD). Chickpea seeds of a Fusarium wilt highly susceptible cultivar JG-62 were surface-sterilized with sodium hypochlorite (2.5% for 5 min) and rinsed with sterilized water (eight times) before being sown into the field. Incidence of Fusarium wilt disease (number of plants showing wilt symptoms to total number of plants in a plot) was recorded on 17, 21, 24 and 28 DAS till the susceptible check showed 100% mortality. Actinomycete population was also enumerated, as explained earlier, from the rhizosphere soils at 28 DAS for all the treatments.





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Subject Agricultural Sciences
Replication number
IAA
Rhizoctonia bataticola
Cellulose
Protaeses
Chickpea
 
Date 2010-10-14
 
Contributor Administrator
 
Type Phenotypic Evaluation
 
Source ICRISAT