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Data on exploring plant growth-promotion actinomycetes from vermicompost and rhizosphere soil for yield enhancement in Chickpea

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Title Data on exploring plant growth-promotion actinomycetes from vermicompost and rhizosphere soil for yield enhancement in Chickpea
 
Identifier https://doi.org/10.21421/D2/GJF2XR
 
Creator Sreevidya M
Gopalakrishnan, S
Kudapa H
Rajeev K. Varshney
 
Publisher ICRISAT Dataverse
 
Description A total of 89 actinomycetes were screened for their antago-nistic activity against S. rolfsii, M. phaseolina (three strains viz.MP-6, MP-24 and MP-115) and FOC by dual culture assay asper the protocols on glucose casamino acid yeast extract agar plates. The culture filtrates of the promising isolates, based on the dual culture assay, were extracted by partitioning against ethyl acetate and the resultant organic and aqueous fractions were evaporated on a rotary evaporator and collected in a minimal volume of methanol. Both the fractions were evaluated for their antagonistic potential against the three fungal pathogens of Chickpea (M. phaseolina, FOC and S. rolfsii). For this, a fungal disc of 6 mm diameter was bored and kept at center of the potato dextrose agar plate amended with either organic or aqueous fractions.
The selected actinomycetes were evaluated for PGP potential on chickpea under on station field conditions in 2013–14 post-rainy season at Chickpea variety ICCV 2 was used for this trial. The actinomycete cultures were grown in SCB at 28 ± 2◦C for 5 days. Chickpea seeds were treated with the actinomycete cultures (108CFU per mL) for 50 min and sown. At 60 DAS, the nodule number, stem weight, pod number, pod weight, leaf weight and leaf area were noted and compared with un-inoculated control. The crop was harvested manually, stover yield, grain yield and total dry matter were noted.





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Subject Agricultural Sciences
Replication number
Dry matter
Leaf area
Plant height
Seed weight
Grain yield
Nitrogen
Phosphorus
Triacylglycerol lipase
Proteases
Chitinase
Hydrocyanic acid
Cellulase
IAA
Chickpea
Pod number
 
Date 2012-11-16
 
Contributor Administrator
 
Type Phenotypic Evaluation
 
Source ICRISAT