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Propensity of bioenrichment protocol in marine ornamental fish larviculture

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Relation http://eprints.cmfri.org.in/14051/
https://shodhganga.inflibnet.ac.in/handle/10603/209709
 
Title Propensity of bioenrichment protocol in marine ornamental fish larviculture
 
Creator Iyapparajanarasimapallavan, G
 
Subject Biochemistry
Ornamental Fishes
 
Description The Marine ornamentals are the most flourishing international business activity, valued at 8
billion US$ with an average annual growth rate of 9% across the world. Even though marine ornamental
fish culture for trade is being practised globally, the early mortality of these altricial fish larvae in captivity
are the major hitch of this sector that occurs due to lack of proper feed or feeding technology. This research
is focused on the propensity of bio-enrichment protocol in marine ornamental fish larviculture using
clownfish larvae (Amphiprion ocellaris) as a model species. Specifically, it was designed to optimize the
feeding protocol of A. ocellaris using live feed enriched with DHA rich oil emulsion and to study their
effects on larval growth and survival under captivity.
The larvae of A. ocellaris were fed with six different diets including the indigenously produced
DHA rich marine pelagic fish oil emulsion, which were delivered through rotifers Brachionus plicatilis.
Locally available low-value oil sardine (Sardinella longiceps) and fish roe (egg) were screened for long chain
n-3 PUFAs and were used as raw material to prepare the PUFA by chemical and/or enzymatic process.
This study has been contemplated for the development of n-3 PUFA enriched formulation based on
different physicochemical properties associated with the olefinic bonds in fatty acids and/or acyl chain
length. The long chain C20-22n-3 PUFAs such as EPA and DHA present in the sardine oil were
concentrated by saponification reaction followed by their enrichment by amide fractionation and
chromatographic methods (the silica gel complexed with a d-block element). The unique substrate specificity
of microbial triacylglycerol acyl hydrolases has also been utilized to further enrich the C20-22 LC PUFAs in
triglycerides.
The microalgal species were isolated from different locations of marine and backwater niches of
Kerala, and their culture conditions in laboratory were optimized. The nutritional properties with respect
to the fatty acid compositions of the purified microalgae at their exponential growth phase were obtained.
The live feed rotifers were cultured in mass by using the shortlisted microalgae, and were further enriched
with indigenously produced DHA rich fish oil emulsion to enhance the nutritional threshold of live feed.
The optimum time period to enrich the live feed by the oil emulsion was described. The marine ornamental
finfish A. ocellaris larvae was fed with the DHA enriched live feed rotifer in different experimental
combinations, and the fatty acid compositions of the larvae were recorded to understand their effect on the
growth and development of the fish larvae. The rotifers (Brachionus plicatilis) were mass cultured in the
presence of DHA rich marine microalgae Isochrysis galbana and further enriched with DHA rich fish oil
emulsion. The fatty acid profile along with the growth and survival of the enriched live fed were compared
with those fed with different commercial diets including a lyophilized algal powder.
 
Date 2015
 
Type Thesis
NonPeerReviewed
 
Format text
 
Language en
 
Identifier http://eprints.cmfri.org.in/14051/1/Thesis_2015_Iyapparajanarasimhapallavan.pdf
Iyapparajanarasimapallavan, G (2015) Propensity of bioenrichment protocol in marine ornamental fish larviculture. Doctoral thesis, ICAR - Central Marine Fisheries Research Institute.