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Establishment and characterisation of cell lines from the caerulean damsel, Pomacentrus caeruleus

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Relation http://eprints.cmfri.org.in/13915/
 
Title Establishment and
characterisation of cell lines from the caerulean damsel, Pomacentrus
caeruleus
 
Creator George, Githa Ann
 
Subject Microbiology
Fish Biotechnology
 
Description Cell lines and primary cell cultures from fish tissues have been used for
research in disease diagnosis and cytotoxicity evaluation of environmental
pollutants. The principal aim of this work was the development of continuous
cell lines from the caerulean damsel, Pomacentus caeruleus. To achieve this,
tissues were first sourced from donor fishes, devoid of contaminants using
standardised disinfection protocols. Primary cultures were then initiated and
suitable media, additives, dissociation reagents as well as optimum incubation
temperature were determined. Successful primary cultures were subcultured
and passaged to derive continuous cell lines which were cryopreserved for
long term storage. The continuous cell lines established were characterised by
immunotyping using cell type markers and authenticated to confirm the
species of origin using previously established barcoding techniques.
Preliminary applications such as gene transfection studies and cytotoxicity
assays using bacterial extracellular products were done in addition to ensuring
that the cultures and cryostocks were contamination-free.
 
Date 2017
 
Type Thesis
NonPeerReviewed
 
Format text
 
Language en
 
Identifier http://eprints.cmfri.org.in/13915/1/Githa%20Ann%20George_2017_Thesis_Pomacentrus%20caeruleus.pdf
George, Githa Ann (2017) Establishment and characterisation of cell lines from the caerulean damsel, Pomacentrus caeruleus. Doctoral thesis, ICAR- Central Marine Fisheries Research Institute.