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Pathological investigations of Vibrio vulnificus infection in Genetically Improved Farmed Tilapia (Oreochromis niloticus L.) cultured at a floating cage farm of India

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Relation http://eprints.cmfri.org.in/13679/
https://www.sciencedirect.com/science/article/pii/S0044848618319240#!
https://doi.org/10.1016/j.aquaculture.2019.734217
 
Title Pathological investigations of Vibrio vulnificus infection in Genetically
Improved Farmed Tilapia (Oreochromis niloticus L.) cultured at a floating
cage farm of India
 
Creator Sumithra, T G
Reshma, K J
Nair, V Anusree
Sayooj, P
Sharma, S R Krupesha
Suja, G
Amala, P V
Joseph, Shoji
Sanil, N K
 
Subject Cage culture
Microbiology
Fish Diseases
 
Description An outbreak of skin ulcers with morbidity and mortality rate of 80% and 20% respectively, among Genetically Improved Farmed Tilapia (GIFT) sub-adults reared in a brackish water floating cage farm of Kochi, Kerala, India was reported. Bacterial isolation attempts from various internal organs (blood, kidney, liver, spleen and brain) of different diseased fish indicated a common bacterial etiology. Organisms were identified as Vibrio vulnificus based on conventional microbiological methods followed by molecular confirmation. Additionally, V. vulnificus marker specific for fish virulence was present in the isolates. Fish pathogenicity was confirmed by challenge tests. LD50 was calculated as 2.1 × 105.13 CFU/fish and 2.1 × 106.12 CFU/mL in injection and immersion route respectively. pilF polymorphism-based PCR and vvhA gene sequence indicated potential danger for humans. The strain was positive for siderophore, hemolysin, capsule, polar and lateral flagella. The strain could multiply rapidly in healthy fish serum. All these results indicated that the isolate could act as a primary pathogen for tilapia fish. Interestingly, serum survival ratio of the pathogen was significantly larger at higher temperatures (p ≤ .05); which may explain the increase in infective capacity of V. vulnificus at higher temperature. Multiple antibiotic resistance index of the strain was ≤0.2. In conclusion, the paper provides detailed descriptions of clinical signs, microscopic and macroscopic lesions of a natural disease outbreak among tilapia raised in brackish water cage farms of India. The concurrent attempts to identify and characterize the etiology fetches the basis for future development of effective control and preventive measures against V. vulnificus, the most infectious and lethal of all zoonotic vibrios.
 
Publisher Elsevier
 
Date 2019
 
Type Article
PeerReviewed
 
Format text
 
Language en
 
Identifier http://eprints.cmfri.org.in/13679/1/Aquaculture_2019_Sumithra%20T%20G_Pathological%20investigations%20of%20Vibrio%20vulnificus%20infection.pdf
Sumithra, T G and Reshma, K J and Nair, V Anusree and Sayooj, P and Sharma, S R Krupesha and Suja, G and Amala, P V and Joseph, Shoji and Sanil, N K (2019) Pathological investigations of Vibrio vulnificus infection in Genetically Improved Farmed Tilapia (Oreochromis niloticus L.) cultured at a floating cage farm of India. Aquaculture, 511. pp. 1-11.