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Enrichment of C20-22 Polyunsaturated Fatty Acids from Refined Liver Oil of Leafscale Gulper Shark, Centrophorus squamosus

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Relation http://eprints.cmfri.org.in/13648/
https://www.tandfonline.com/doi/abs/10.1080/10498850.2017.1375588
https://doi.org/10.1080/10498850.2017.1375588
 
Title Enrichment of C20-22 Polyunsaturated Fatty Acids from Refined
Liver Oil of Leafscale Gulper Shark, Centrophorus squamosus
 
Creator Joseph, Dexy
Chakraborty, Kajal
 
Subject Bioprospecting
Bioactive compounds
Biochemistry
Fish Biotechnology
 
Description The C20-22 polyunsaturated fatty acids (C20-22 PUFAs), mainly eicosapentaenoic acid (EPA, 20:5n-3) and docosahexaenoic acid (DHA, 22:6n-3), were concentrated from the refined liver oil of deep sea leafscale gulper shark, Centrophorus squamosus, by sequential processes of winterization, urea complexation, and argentation chromatography. Winterization at 4°C using acetonitrile as solvent showed significant reduction of the total saturated fatty acid content (12%) with a concomitant increase of polyunsaturated fatty acids (36.3%). The urea complexation process significantly enriched the total polyunsaturated fatty acids (49.5%), with a reduction in saturated fatty acids (83.9%). Silica gel based argentation chromatography further concentrated the esters of C20-22 PUFA (>99 percent purity). Nuclear Magnetic Resonance spectroscopy was used as a chemical fingerprinting tool to study the enrichment of C20-22 PUFAs at various stages of the purification process. An increase in the proton signal intensity at the olefinic region (at δ 4–6) and a decrease in the aliphatic signals (at δ 0.5–2) showed that the process successfully enriched the C20-22 PUFAs.
 
Publisher Elsevier
 
Date 2017
 
Type Article
PeerReviewed
 
Format text
 
Language en
 
Identifier http://eprints.cmfri.org.in/13648/1/Journal%20of%20Aquatic%20Food%20Product%20Technology_2017_Kajal%20Chakraborty_Enrichment%20of%20C20-22%20polyunsaturated%20fatty%20acids%20from%20refined%20liver%20oil%20of%20Leafscale%20Gulper%20Shark.pdf
Joseph, Dexy and Chakraborty, Kajal (2017) Enrichment of C20-22 Polyunsaturated Fatty Acids from Refined Liver Oil of Leafscale Gulper Shark, Centrophorus squamosus. Journal of Aquatic Food Product Technology, 26 (9). pp. 1042-1056.