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Dual activities of receptor-like kinase OsWAKL21.2 induce immune responses

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Title Dual activities of receptor-like kinase OsWAKL21.2 induce immune responses
 
Creator Malukani, Kamal Kumar
Ranjan, Ashish
Hota, Shiva Jyothi
Patel, Hitendra Kumar
Sonti, Ramesh V.
 
Subject Receptor-Like Kinase
Oryza sativa
Induce Immune Responses
rice
 
Description Accepted date: April 14, 2020
Plant pathogens secrete cell wall-degrading enzymes that degrade various components of the plant cell wall. Plants sense this
cell wall damage as a mark of infection and induce immune responses. However, the plant functions that are involved in the
elaboration of cell wall damage-induced immune responses remain poorly understood. Transcriptome analysis revealed that a
rice (Oryza sativa) receptor-like kinase, WALL-ASSOCIATED KINASE-LIKE21 (OsWAKL21.2), is up-regulated following
treatment with either Xanthomonas oryzae pv oryzae (a bacterial pathogen) or lipaseA/esterase (LipA; a cell wall-degrading
enzyme of X. oryzae pv oryzae). Overexpression of OsWAKL21.2 in rice induces immune responses similar to those activated
by LipA treatment. Down-regulation of OsWAKL21.2 attenuates LipA-mediated immune responses. Heterologous expression of
OsWAKL21.2 in Arabidopsis (Arabidopsis thaliana) also activates plant immune responses. OsWAKL21.2 is a dual-activity kinase
that has in vitro kinase and guanylate cyclase activities. Interestingly, kinase activity of OsWAKL21.2 is necessary to activate rice
immune responses, whereas in Arabidopsis, OsWAKL21.2 guanylate cyclase activity activates these responses. Our study
reveals a rice receptor kinase that activates immune responses in two different species via two different mechanisms.
We thank Ramesh Palaparthi (Council of Scientific and Industrial Research, Centre for Cellular and Molecular Biology) for helping in analyzing
the microarray data; Dr. Alok K. Sinha (Department of Biotechnology,
National Institute of Plant Genome Research), Dr. Gopaljee Jha (Department
of Biotechnology, National Institute of Plant Genome Research), and Dr.
Puran Singh Sijwali (Council of Scientific and Industrial Research-Centre for
Cellular and Molecular Biology) for their key suggestions in experiments; and
Dr. Subhadeep Chatterjee (Department of Biotechnology, Centre for DNA
Fingerprinting and Diagnostics) for providing NahG transgenic lines and the
Pst DC3000 strain.
 
Date 2020-07-13T07:05:28Z
2020-07-13T07:05:28Z
2020
 
Type Article
 
Identifier Plant Physiology, 183(3): 1345-1363
1532-2548
https://doi.org/10.1104/pp.19.01579
http://www.plantphysiol.org/content/183/3/1345
http://223.31.159.10:8080/jspui/handle/123456789/1072
 
Language en_US
 
Format application/pdf
 
Publisher American Society of Plant Biologists