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Calcium channel CNGC19 mediates basal defense signaling to regulate colonization of Piriformospora indica in Arabidopsis roots

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Title Calcium channel CNGC19 mediates basal defense signaling to regulate colonization of Piriformospora indica in Arabidopsis roots
 
Creator Jogawat, Abhimanyu
Meena, Mukesh Kumar
Kundu, Anish
Varma, Mahendra
Vadassery, Jyothilakshmi
 
Subject Piriformospora indica
Serendipita indica
CNGC19
indole glucosinolates
callose
cell wall extract
cellotriose
 
Description Accepted date: 16 January 2020
The activation of calcium signaling is a crucial event for perceiving environmental stress. Colonization by Piriformospora indica, a growth promoting root endosymbiont activates cytosolic Ca2+ in Arabidopsis roots. In this study, we analyze the role of calcium channels responsible for Ca2+ fluxes and its functional relevance. Expression profiling revealed that CNGC19 is a early activated gene, induced by unidentified components in P. indica cell wall extract. Functional analysis revealed that loss-of-function of CNGC19 results in growth inhibition by P.indica, due to increased colonization and loss of controlled P. indica growth. P. indica cell wall extract induced cytosolic Ca2+ elevation is reduced in cngc19 mutant indicating a role in generation of Ca2+cyt elevation. MAMP-trigerred immunity (MTI) is compromised in cngc19 lines as evident from unaltered callose deposition, reduced cis-OPDA, JA and JA-Ile levels and downregulation of jasmonate and other defense related genes which contributes to shift towards pathogenic response. Loss-of-function of CNGC19 results in inability to modulate indole glucosinolate content during P. indica-colonization. CNGC19 mediated basal immunity is AtPep receptor, PEPR dependent. CNGC19 is also crucial for P. indica mediated suppression of AtPep induced immunity. Thus, Arabidopsis CNGC19 is an important Ca2+ channel, maintaining a robust innate immunity and crucial for growth promotion signalling upon P. indica colonization.
We thank Ralf Oelmüller, Friedrich Schiller University, Jena, Germany for gifting GFPtagged P. indica, Michael Reichelt (Max Planck Institute for Chemical Ecology, Jena) for
phytohormone quantification help. AJ acknowledges Scientific and Engineering Research
Board (SERB), Government of India for providing fellowship and research funding through
the National Postdoctoral fellowship scheme. JV, AJ, MKM, AK and MV acknowledge
Department of Biotechnology (DBT), NIPGR, central instrumentation, metabolome and
phytotron facility.
 
Date 2020-01-24T09:01:19Z
2020-01-24T09:01:19Z
2020
 
Type Article
 
Identifier Journal of Experimental Botany, 71(9): 2752-2768
1460-2431
https://doi.org/10.1093/jxb/eraa028
https://academic.oup.com/jxb/advance-article/doi/10.1093/jxb/eraa028/5709769
http://223.31.159.10:8080/jspui/handle/123456789/1033
 
Language en_US
 
Format application/pdf
 
Publisher Oxford University Press