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Effects of miR-222 on cisplatin resistance of renal cancer cell strains and related mechanisms

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Title Statement Effects of miR-222 on cisplatin resistance of renal cancer cell strains and related mechanisms
 
Added Entry - Uncontrolled Name Liu, Haozhi
Zhang, Yun
 
Uncontrolled Index Term Dickkopf-3; Kidney; microRNAs; PCR; Proliferation
 
Summary, etc. Cisplatin is widely employed in combating renal cancers. However, a major challenge is the resistance of cisplatin by renal cancer cells. Increased miR-222 expression is known to promote this resistance and so ways of downregulating the expression of miR-222 is widely sought after as a means of combating the resistance. The study was performed to explore the effects of miR-222 on cisplatin resistance of renal cancer cell strains and related mechanisms. The expression of miR-222 and Dickkopf-3 (DKK3) was regulated to explore the effects of miR-222 and DKK3 on cisplatin chemotherapy in renal cancer cisplatin-resistant cell strains, and to figure out the regulatory relationship between miR-222 and DKK3. In renal cancer tissues and cell lines, miR-222 was highly expressed and DKK3 was lowly expressed. Renal cancer cisplatin-resistant cells had markedly higher miR-222 expression and lower DKK3 expression than normal renal cancer cells, suggesting a potential involvement of miR-222 and DKK3 in the cisplatin resistance in renal cancer cells. Down-regulated miR-222 expression led to stronger inhibition of the renal cancer cell growth by cisplatin and markedly higher DKK3 expression. Dual-luciferase reporter assay results showed that miR-222 had a targeted inhibition on DKK3. Co-transfection of miR-222 mimic and shDKK3, together with the up-regulation of miR-222 and DKK3 expressions, resulted in a higher sensitivity of renal cancer cells to cisplatin chemotherapy than the up-regulation of miR-222 expression alone. Down-regulated miR-222 expression can remove the inhibition of DKK3 expression and increase the sensitivity of renal cancer cells to cisplatin chemotherapy.
 
Publication, Distribution, Etc. Indian Journal of Biochemistry and Biophysics (IJBB)
2020-07-29 16:48:33
 
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http://op.niscair.res.in/index.php/IJBB/article/view/31805
 
Data Source Entry Indian Journal of Biochemistry and Biophysics (IJBB); ##issue.vol## 57, ##issue.no## 4 (2020): IJBB Vol.57(4) [August 2020]
 
Language Note en