Record Details

Diversity of <em>Bacillus thuringiensis cry</em> genes in soils of Andhra Pradesh, India

Online Publishing @ NISCAIR

View Archive Info
 
 
Field Value
 
Authentication Code dc
 
Title Statement Diversity of <em>Bacillus thuringiensis cry</em> genes in soils of Andhra Pradesh, India
 
Added Entry - Uncontrolled Name Kayam, Devaki
Tirupati, Murali Krishna
Karanam, Hari Prasad
 
Uncontrolled Index Term Bacillus thuringiensis strains; Cry gene; Southern zone; Spodoptera litura; δ-endotoxins PCR
 
Summary, etc. A total of 61 bacterial strains was isolated in soils from Chittoor and Kadapa districts of Andhra Pradesh, India using sodium acetate selection method and preliminarily identified as Bacillus thuringiensis based on Gram's staining, endospore staining and crystal protein stud. These strains were tested against the insect, Spodoptera litura under laboratory conditions and found 18 strains with ≥ 50% insect mortality. Further, these eighteen strains were analyzed for the insecticidal crystal protein composition with fourteen cry specific primers. The results indicated that, among all 14 cry genes, cry1I was the observed in 11 strains, followed by cry1Aa in 10 strains. At the same time a strain, F493 collected from Talakona area identified as effective against S. litura with 100 per cent larval death and which was also identified with 8 different crystal protein encoding genes (cry1Aa, cry1Ac, cry1Fa1, cry1I, cry2, cry2A(a)1, cry8, cry9Ca1).
 
Publication, Distribution, Etc. Indian Journal of Biochemistry and Biophysics (IJBB)
2020-07-29 16:48:33
 
Electronic Location and Access application/pdf
http://op.niscair.res.in/index.php/IJBB/article/view/25815
 
Data Source Entry Indian Journal of Biochemistry and Biophysics (IJBB); ##issue.vol## 57, ##issue.no## 4 (2020): IJBB Vol.57(4) [August 2020]
 
Language Note en