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<p class="TitleMain" style="text-align: justify;">UV Induced Mutagenesis Elevates the Production of Laccase in <em>Enterobacter cloacae</em><em></em></p>

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Title Statement <p class="TitleMain" style="text-align: justify;">UV Induced Mutagenesis Elevates the Production of Laccase in <em>Enterobacter cloacae</em><em></em></p>
 
Added Entry - Uncontrolled Name Deepa, T ; Faculty of Applied Sciences, Parul University, Vadodara, Gujarat, India
Gangwane, A K; Faculty of Applied Sciences, Parul University, Vadodara, Gujarat, India
Sayyed, R Z; Department of Microbiology, PSGVP Mandal’s Arts, Science, and Commerce College, Shahada Maharashtra 425409, India
., Sapna ; ICAR-National Bureau of Plant Genetic Resources, Pusa Campus, New Delhi 110012, India
El Enshasy, H A; Institute of Bioproduct Development (IBD), Universiti Teknologi Malaysia (UTM), Johor Bahru, Malaysia
Zaidel, D N A; School of Chemical and Energy Engineering, Faculty of Engineering, Universiti Teknologi Malaysia, Johor Bahru, Malaysia
 
Uncontrolled Index Term Enterobacter cloacae; Laccase production; Optimization; UV mutation
 
Summary, etc. <p class="Abstract">The increasing commercial significance of microbial laccase in various fields compels the supply of enzymes in large quantities at an affordable price. Laccase has been produced by a wide variety of wild cultures of bacteria and fungi, the high yielding strains are always desired to meet the increasing demand of laccase. Although various approaches have been proposed for improving the yield of laccase, UV mutagenesis is known as the best method of improving the strains for better yield of microbial metabolites. However, the reports on the use of UV light in strain improvement for laccase production by the use of <em>Enterobacter</em> sp. are scarce and need to be explored fully. For this purpose present study was aimed to improve the laccase production in <em>Enterobacter</em> <em>cloacae </em>through UV mutagenesis. We report enhanced production of laccase in a high yielding strain of <em>E. cloacae </em>isolated from the soap industry waste. UV mutagenesis of a wild strain of <em>E. cloacae </em>resulted in 3.09 folds further improvement in the production. Optimum laccase synthesis was reported at 26 h, of incubation at 30ºC under neutral pH (7.0) conditions. This high yielding mutant strain will have great industrial significance for laccase production.</p>
 
Publication, Distribution, Etc. Journal of Scientific and Industrial Research (JSIR)
2020-07-29 12:49:53
 
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http://op.niscair.res.in/index.php/JSIR/article/view/38453
 
Data Source Entry Journal of Scientific and Industrial Research (JSIR); ##issue.vol## 79, ##issue.no## 05
 
Language Note en