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Calcium regulates the mycophagous ability of Burkholderia gladioli strain NGJ1 in a type III secretion system-dependent manner

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Title Calcium regulates the mycophagous ability of Burkholderia gladioli strain NGJ1 in a type III secretion system-dependent manner
 
Creator Yadav, Sunil Kumar
Das, Joyati
Kumar, Rahul
Jha, Gopaljee
 
Subject Mycophagy
Bacterial-fungal interaction
T3SS
Effectors
Endo-β-1
3- glucanase
 
Description Accepted date: 12 July 2020
Background: A rice associated bacterium Burkholderia gladioli strain NGJ1 demonstrates mycophagy, a phenomenon
wherein bacteria feed on fungi. Previously, we have reported that NGJ1 utilizes type III secretion system (T3SS) to
deliver a prophage tail-like protein (Bg_9562) into fungal cells to establish mycophagy.
Results: In this study, we report that calcium ion concentration influences the mycophagous ability of NGJ1 on
Rhizoctonia solani, an important fungal pathogen. The calcium limiting condition promotes mycophagy while high
calcium environment prevents it. The expression of various T3SS apparatus encoding genes of NGJ1 was induced and
secretion of several potential T3SS effector proteins (including Bg_9562) into extracellular milieu was triggered under
calcium limiting condition. Using LC-MS/MS proteome analysis, we identified several calcium regulated T3SS effector
proteins of NGJ1. The expression of genes encoding some of these effector proteins was upregulated during
mycophagous interaction of NGJ1 with R. solani. Further, mutation of one of these genes (endo-β-1, 3- glucanase)
rendered the mutant NGJ1 bacterium defective in mycophagy while complementation with full length copy of the
gene restored its mycophagous activity.
Conclusion: Our study provides evidence that low calcium environment triggers secretion of various T3SS effectors
proteins into the extracellular milieu and suggests the importance of cocktail of these proteins in promoting
mycophagy
We acknowledge the central research facility (CRF) of IIT- Delhi and metabolomic
facility of NIPGR (DBT grant no. BT/INF/22/SP28268/2018), New Delhi for help in ICPMS analysis. The authors are also thankful to DBT-eLibrary Consortium (DelCON) for
providing access to e-resources. The assistance of NIPGR central instrumentation facilities for sequencing and qRT-PCR are acknowledged. We sincerely thank Dr. Ramesh
V. Sonti and Dr. Srayan Ghosh for comments on the manuscript.
 
Date 2020-07-28T10:22:27Z
2020-07-28T10:22:27Z
2020
 
Type Article
 
Identifier BMC Microbiology 20(1): 216
1471-2180
https://doi.org/10.1186/s12866-020-01897-2
https://bmcmicrobiol.biomedcentral.com/articles/10.1186/s12866-020-01897-2
http://223.31.159.10:8080/jspui/handle/123456789/1079
 
Language en_US
 
Format application/pdf
 
Publisher BioMed Central Ltd