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MsrA Efflux Pump Inhibitory Activity ofPiper cubebaL.f. and its Phytoconstituents againstStaphylococcus aureusRN4220

DIR@IMTECH: CSIR-Institute of Microbial Technology

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Title MsrA Efflux Pump Inhibitory Activity ofPiper cubebaL.f. and its Phytoconstituents againstStaphylococcus aureusRN4220
 
Creator Ahirrao, Pallavi
Tambat, Rushikesh
Chandal, Nishtha
Mahey, Nisha
Kamboj, Anjoo
Jain, Upendra K.
Singh, Inder Pal
Jachak, Sanjay M.
Nandanwar, Hemraj
 
Subject QR Microbiology
 
Description MsrA, an efflux pump belonging to ATP-binding cassette (ABC) transporter family that conferred resistance to macrolides, was detected inStaphylococcus aureusstrains. Herein, we report the isolation of phytoconstituents fromPiper cubebafruit methanol extract and investigated their efflux pump inhibitory potential againstS. aureusMsrA pump. Four isolated compounds, viz. pellitorine, sesamin, piperic acid and tetrahydropiperine studied in combination with erythromycin inS. aureusRN4220, exhibited 2-8-fold reduction in minimum inhibitory concentration (MIC) of erythromycin. Pellitorine and sesamin decreased MIC of erythromycin by 8-fold. The real-time fluorometry-based efflux and accumulation studies of ethidium bromide (EtBr) onS. aureusRN4220 in the presence of these compounds showed reduced efflux and enhanced uptake, thus indicating inhibition of the efflux pump. Pellitorine showed significant post-antibiotic effect of erythromycin. The results revealed that the primary mechanism of action of these compounds involves steady ATP production impairment.
 
Publisher WILEY-V C H VERLAG GMBH
 
Date 2020-07-05
 
Type Article
PeerReviewed
 
Relation https://onlinelibrary.wiley.com/doi/full/10.1002/cbdv.202000144
http://crdd.osdd.net/open/2584/
 
Identifier Ahirrao, Pallavi and Tambat, Rushikesh and Chandal, Nishtha and Mahey, Nisha and Kamboj, Anjoo and Jain, Upendra K. and Singh, Inder Pal and Jachak, Sanjay M. and Nandanwar, Hemraj (2020) MsrA Efflux Pump Inhibitory Activity ofPiper cubebaL.f. and its Phytoconstituents againstStaphylococcus aureusRN4220. Chemistry & Biodiversity. (In Press)