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Chromatographic separation of sugars

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Relation http://eprints.cmfri.org.in/3271/
 
Title Chromatographic separation of sugars
 
Creator Saravanan, T S
Arumugam, M
 
Subject Crustacean Fisheries
Biochemical Study
 
Description The principle of chromatography involves separation of a
mixture on the basis of specific differences in physical and chemical properties, which result from the structural
differences of the chemically related groups of compounds which are under investigation. They therefore have differential affinity for both the mobile and stationary phases of the chromatographic systems. This chromatographic separation is the resultant of
propelling (mobile phase) and retarding forces (stationary phase). The stationary phase in strict sense includes the medium (paper) together with the polar solvent (water). The mobile phase or propelling force includes both polar and non-polar solvent. The separation is brought about by continuous partition between the mobile phase (solvent flowing along the paper) and the water held in the paper and paper per se. Paper together with water acts as an adsorbent; it has a strong affinity for polar molecules which are held by hydrogen bonding and vander Waals' forces (Smith & Seakins, 1976).
 
Publisher CMFRI
 
Date 1981
 
Type Article
PeerReviewed
 
Format application/pdf
 
Language en
 
Identifier http://eprints.cmfri.org.in/3271/1/Spl_7-16.pdf
Saravanan, T S and Arumugam, M (1981) Chromatographic separation of sugars. CMFRI Special Publication (7). pp. 117-119.