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High resolution mapping of restoration of fertility (Rf) by combining large population and high density genetic map in pigeonpea [Cajanus cajan (L.) Millsp]

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Relation http://oar.icrisat.org/11545/
https://doi.org/10.1186/s12864-020-06859-6
doi:10.1186/s12864-020-06859-6
 
Title High resolution mapping of restoration of fertility (Rf) by combining large population and high density genetic map in pigeonpea [Cajanus cajan (L.) Millsp]
 
Creator Saxena, R K
Molla, J
Yadav, P
Varshney, R K
 
Subject Pigeonpea
Genetics and Genomics
 
Description Background: Restoration of fertility (Rf) is an important trait for pigeonpea hybrid breeding. Few coarse
quantitative trait locus (QTL) studies conducted in the past identified QTLs with large confidence intervals on the
genetic map and could not provide any information on possible genes responsible for Rf in pigeonpea. Therefore, a
larger population comprising of 369 F2s derived from ICPA 2039 × ICPL 87119 was genotyped with high density
Axiom Cajanus SNP Array with 56 K single nucleotide polymorphism (SNPs) for high resolution mapping of Rf.

Results: A genetic map with 4867 markers was developed and a total of four QTLs for Rf were identified. While one
major effect QTL (qRf8.1) was co-localized with the QTL identified in two previous studies and its size was refined
from 1.2 Mb to 0.41 Mb. Further analysis of qRf8.1 QTL with genome sequence provided 20 genes including two
genes namely flowering locus protein T and 2-oxoglutarate/Fe (II)-dependent dioxygenases (2-ODDs) superfamily
protein with known function in the restoration of fertility.

Conclusion: The qRf8.1 QTL and the potential candidate genes present in this QTL will be valuable for genomicsassisted
breeding and identification of causal genes/nucleotides for the restoration of fertility in the hybrid
breeding program of pigeonpea.
 
Publisher BMC
 
Date 2020-07
 
Type Article
PeerReviewed
 
Format application/pdf
 
Language en
 
Identifier http://oar.icrisat.org/11545/1/s12864-020-06859-6.pdf
Saxena, R K and Molla, J and Yadav, P and Varshney, R K (2020) High resolution mapping of restoration of fertility (Rf) by combining large population and high density genetic map in pigeonpea [Cajanus cajan (L.) Millsp]. BMC Genomics (TSI), 21 (1). pp. 1-8. ISSN 1471-2164